GeneChip™ Human Genome U133 Plus 2.0 Array

GeneChip™ Human Genome U133 Plus 2.0 ArrayBenefits of the first and most comprehensive whole human genome expression array• Convenient one-arrayRead more

Catalog Number	Number of Arrays
900467	30 arrays

Catalog number 900467

Price (CNY)

Number of Arrays:

30 arrays

GeneChip™ Human Genome U133 Plus 2.0 Array

Benefits of the first and most comprehensive whole human genome expression array
• Convenient one-array view.
• Complete coverage of the Human Genome U133 Set plus 6,500 additional genes for analysis of over 47,000 transcripts.
• Use the Power of the Probe Set and get multiple independent measurements for each transcript that deliver the greatest accuracy and reproducibility of any microarray platform.
• GeneChip System: Richer data, sharper insights, better decisions

Array Profile
All probe sets represented on the GeneChip Human Genome U133 Set are identically replicated on the GeneChip Human Genome U133 Plus 2.0 Array. The sequences from which these probe sets were derived were selected from GenBank™, dbEST, and RefSeq. The sequence clusters were created from the UniGene database (Build 133, April 20, 2001) and then refined by analysis and comparison with a number of other publicly available databases, including the Washington University EST trace repository and the University of California, Santa Cruz Golden-Path human genome database (April 2001 release).

In addition, there are 9,921 probe sets representing approximately 6,500 genes. These gene sequences were selected from GenBank, dbEST, and RefSeq. Sequence clusters were created from the UniGene database (Build 159, January 25, 2003) and refined by analysis and comparison with a number of other publicly available databases, including the Washington University EST trace repository and the NCBI human genome assembly (Build 31).

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product LineGeneChip™

Quantity30 arrays

TypeHuman Genome U133 Plus 2.0 Array

ArrayTranscriptome Profiling

FormatArray Cartridge

Number of Arrays30 arrays

SpeciesHuman

Unit SizeEach

Frequently asked questions (FAQs)

What reagent kit should I use with my array?

Please refer to the Microarray Reagent Guide for Arrays and Expression Kits to match the correct reagents your array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How are transcript IDs assigned for WT arrays if a gene has multiple transcript IDs?

The first assigned mRNA/gene that is listed in the annotation file for a given probe set or transcript cluster is considered the "best" assignment based on data source quality ranking and assignment scoring system. To get a single best mRNA/gene assignment you can simply take the first one in the list in the annotation file.

Sometimes, there might be multiple best hits between transcript clusters (or probe set) and a mRNA/gene, with identical score and data source quality. In that case, the transcript assignments are ranked based on their descriptions (e.g., title from the GenBank record).

For transcript clusters that detect genes that are alternatively spliced, the different spliced forms will be ranked by length with the longest ones listed first.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Does the GeneChip miRNA 3.1 Array Strip require a different hyb/wash/stain kit than the GeneChip 3' IVT arrays and GeneChip ST array strips?

The hyb/wash/stain kit for the GeneChip miRNA 3.1 Array Strips is different than that for the 3' IVT arrays and GeneChip ST array strips. Additional wash A steps have been added to ensure adequate washing after the array strips have been stained.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What do the suffixes mean at the end of each probeset in GeneChip 3' IVT arrays?

The probe set names never change, but they can give you an idea of what was known about the sequence at the time of design.
_at: All probes hit one known transcript.
_a: All probes in the set hit alternate transcripts from the same gene.
_s: All probes in the set hit transcripts from different genes.
_x: Some probes hit transcripts from different genes.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Can I store my GeneChip 3' IVT Array after hybridization?

Yes, but we recommend storing your GeneChip 3' IVT Array after hybridization and prior to the fluidics step, for no longer than 3 hours at 4 degrees C. Please remove the hybridization cocktail and fill with wash A before storing.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

View all GeneChip™ Human Genome U133 Plus 2.0 Array, 30 arrays FAQs