Pierce™ Glu-C Protease, MS Grade, 5 x 10 μg - FAQs

What is the best enzyme to use for protein digestion for mass spectrometry samples?

Trypsin (Cat. Nos. 90057, 90058) or Trypsin/LysC mix (Cat. Nos. A40007, A40009, A41007) are most commonly used for proteomic applications in order to ensure reproducibility and complete digestion. Other commonly used enzymes for purified protein characterization and unique applications include Chymotrypsin Protease, MS Grade (Cat. No. 90056), Immobilized Pepsin (Cat. No. 20343), LysN Protease, MS Grade (Cat. No. 90300), Asp-N Protease, MS Grade (Cat. No. 90053), and Glu-C Protease, MS Grade (Cat. No. 90054).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.