Pierce™ Trypsin Protease, MS Grade, 5 x 100 μg - FAQs

查看更多产品信息 Mass Spectrometry Grade Proteases - FAQs (90054, A40009, 90057, 90056, 90059, A40007, 90058, A41007, 90051, 90053, 90305, 90307)

2 个常见问题解答

What is the best enzyme to use for protein digestion for mass spectrometry samples?

Trypsin (Cat. Nos. 90057, 90058) or Trypsin/LysC mix (Cat. Nos. A40007, A40009, A41007) are most commonly used for proteomic applications in order to ensure reproducibility and complete digestion. Other commonly used enzymes for purified protein characterization and unique applications include Chymotrypsin Protease, MS Grade (Cat. No. 90056), Immobilized Pepsin (Cat. No. 20343), LysN Protease, MS Grade (Cat. No. 90300), Asp-N Protease, MS Grade (Cat. No. 90053), and Glu-C Protease, MS Grade (Cat. No. 90054).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can you please recommend a method for reduction, alkylation, and digestion of an antibody?

Prepare 1 mg/mL antibody in 7.0 M Guanidine HCl, 100 mM Tris, pH 8.3. For reduction, add DTT (Cat. Nos. A39255, 20290) solution at 10 mM final concentration and incubate for 30 mins at room temperature. For alkylation, add sodium iodoacetate at 20 mM final concentration and incubate in the dark for 20 mins at room temperature. Perform buffer exchange followed by digestion using Pierce MS grade Trypsin, Cat. Nos. 90058, 90057 (1:10 enzyme to substrate ratio).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.