TMTduplex™ Isobaric Mass Tagging Kit - FAQs

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3 个常见问题解答

What type of mass spectrometer can I use for Tandem Mass Tag (TMT) analysis?

We recommend using high-resolution Orbitrap Tribrid (e.g., Fusion, Lumos, Eclipse), Orbitrap Exploris (e.g., 240, 480), or Q Exactive (e.g,. Plus, HF, HFX) series of instruments.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Is TMT quantitation relative or absolute?

TMT quantitation is relative between samples unless a heavy peptide standard is included for comparison.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the main purpose of TMT (Tandem Mass Tag) reagents?

TMT technology is used to perform multiplex quantitation of proteins extracted from cells and tissues. Rather than quantify single proteins (for example with an antibody), here, large numbers of proteins can be quantified in a single run.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.