GeneChip™ WT 末端标记试剂盒
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Applied Biosystems™

GeneChip™ WT 末端标记试剂盒

GeneChip™ WT 有义靶标标记和对照试剂 (900652) 和 GeneChip WT cDNA 合成和扩增试剂盒（900672了解更多信息
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货号反应次数
90067130 Reactions
90067010 Reactions
货号 900671
价格（CNY)
1,000,000,000.00
Each
添加至购物车
反应次数:
30 Reactions
价格（CNY)
1,000,000,000.00
Each
添加至购物车
GeneChip™ WT 有义靶标标记和对照试剂 (900652) 和 GeneChip WT cDNA 合成和扩增试剂盒（900672 和 900673）在 2010 年 6 月停产。

全转录本 (WT) 有义靶标标记检测试剂盒具有更新的方案。GeneChip WT（全转录本）末端标记试剂盒经过优化，可用于 GeneChip WT 有义靶标 (ST) 标记检测的片段化和标记步骤。

WT 末端标记试剂盒利用一种策略，将尿嘧啶 DNA 糖基化酶 (UDG) 和脱嘌呤/脱嘧啶核酸内切酶 1 (APE 1) 结合在一起，用于可重复的 DNA 片段化。典型的输入材料是随机引物单链 DNA

片段化后，然后可在存在专有的生物素化化合物（GeneChip DNA 标记试剂）的情况下，用末端脱氧核苷酸转移酶 (TdT) 标记得到的片段化 DNA。这两组分也在 WT 末端标记试剂盒中提供。终末标记的 DNA 靶标可添加到混合物中，用于杂交至阵列，例如 GeneChip 人类外显子 1.0 ST 阵列。
仅供科研使用。不可用于诊断程序。
规格
包括标签或染料直接标记
反应次数30 Reactions
产品线GeneChip™
产品类型WT 末端标记试剂盒
数量30 reactions
检测方法基于生物素
最终产品类型DNA（片段化、标记）
产品规格试剂盒
标记目标随机引物 DNA（单链）
Unit SizeEach

常见问题解答 (FAQ)

How long does it take to scan an array?

It takes approximately 35 minutes to scan each Exon Array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Can I use the WT Sense Target Labeling Assay protocol for prokaryotic arrays?

This has not been tested at the moment; therefore, it is not recommended to use the protocol for any application other than on the Exon Arrays.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What is the typical cRNA yield after the IVT reaction in the first cycle?

Starting with 1 µg of total RNA, following the standard protocol, ≥ 16 µg of cRNA is routinely obtained. Starting with 100 ng of total RNA without the RiboMinus rRNA removal step, typically, 20 to 60 µg of cRNA can be generated after cRNA cleanup.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Why do you choose to use an rRNA-reduction strategy but not a poly-A mRNA-specific selection protocol?

It has been shown that a portion of the transcripts in total RNA do not necessarily contain Poly-A tails; therefore, they will be excluded by a poly-A RNA positive selection technique. The rRNA reduction approach will also make the protocol more robust in handling smaller amounts of total RNA samples as little as 1 µg.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Why is Betaine added to the RiboMinus Hybridization Buffer?

Betaine increases the hybridization stringency. It equalizes the GC Tm and AT Tm, so the background, non-specific, non-rRNA hybridization to the RiboMinus probes due to high GC content may be reduced.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

View all GeneChip™ WT 末端标记试剂盒 FAQs