溶菌酶溶液 (50 mg/mL)
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溶菌酶溶液 (50 mg/mL)
Thermo Scientific™

溶菌酶溶液 (50 mg/mL)

Thermo Scientific 溶菌酶是一种酶,其特征在于能够破坏细菌细胞壁以提高蛋白或核酸提取效率。甘油冻存溶菌酶的特点:•细菌细胞壁溶解酶可改善包涵体蛋白纯化•与 Thermo Scientific Pierce 细胞裂解试剂兼容•透明无色,不含不溶性材料•pH 值:4.7 ±0.2•了解更多信息
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货号数量
900820.5 mL
货号 90082
价格(CNY)
870.00
Each
添加至购物车
数量:
0.5 mL
请求批量或定制报价
价格(CNY)
870.00
Each
添加至购物车
Thermo Scientific 溶菌酶是一种酶,其特征在于能够破坏细菌细胞壁以提高蛋白或核酸提取效率。

甘油冻存溶菌酶的特点:

•细菌细胞壁溶解酶可改善包涵体蛋白纯化
•与 Thermo Scientific Pierce 细胞裂解试剂兼容
•透明无色,不含不溶性材料
•pH 值:4.7 ±0.2
• ≥50 单位/mL (5 µg/mL)

溶菌酶是一种可裂解细菌细胞壁以提高蛋白或核酸提取效率的酶。溶解酵素(溶菌酶)是一个有抗菌活性的酶族,可破坏细菌细胞壁。这种酶可催化肽聚糖中 N-乙酰胞壁酸和 N-乙酰-D-葡萄糖胺残基间以及壳糊精中 N-乙酰-D-葡萄糖胺残基间的 1,4-β-连键的水解。尽管鸡蛋清溶菌酶对革兰氏阳性菌的裂解极为有效,但它还有助于革兰氏阴性细菌的裂解,如沙门氏菌属志贺氏菌属大肠杆菌的裂解通过添加溶菌酶和核酸酶(如 DNase I)而得到特别改善。

溶菌酶的描述和特性:
溶菌酶自然存在于植物和动物组织以及眼泪、唾液和粘液等分泌物中;其在蛋清中的含量尤其丰富,是商业用品的主要来源。鸡蛋清溶菌酶(鸡型和 c 型)是第一种 2-埃晶体结构得到解析的酶,并且已被广泛研究。溶解酵素被广泛用于细菌蛋白抽提。对于包涵体纯化,添加溶解酵素以消化细胞碎片并释放包涵体。在对靶标重组蛋白进行融合标签特定亲和纯化以形成包涵体期间,可很容易地将这种酶与其他蛋白一起去除。

•酶功能:催化肽聚糖中 N-乙酰胞壁酸和 N-乙酰-D-葡萄糖胺残基间以及壳糊精中 N-乙酰-D-葡萄糖胺残基间的 1,4-β-连键的水解
•分子量:14.388 kDa
•消光系数:280 nm 处 ∼26.4
• 等电点:pH 值 11.0
• 单位定义:1 单位是在 25 摄氏度、pH 值为 6.24 的环境下,在 1 cm 比色皿中因使用 ∼0.25 mg/mL 藤黄微球菌悬浮液裂解催化 450 nm 处吸光度下降 0.001/min 所需的酶量。

相关产品
溶菌酶
仅供科研使用。不可用于诊断程序。
规格
溶菌酶(胞壁酸酶)
数量0.5 mL
试剂类型用于细胞裂解的酶
形式液体
产品类型裂解液
Unit SizeEach
内容与储存
在低于 -20°C 下储存

常见问题解答 (FAQ)

How can I measure the amount of protein I have extracted with B-PER reagent?

The Thermo Scientific BCA Protein Assay Kit (Cat. No. 23225, 23227) works very well to detect proteins extracted with B-PER Reagent. The Coomassie Plus Protein Assay (Cat. No. 23236) can be used with B-PER Reagent Protein extractions, however when using B-PER with PBS or B-PER II reagents, dilute the protein preparation two-to-four fold before assaying with Coomassie Plus or Prepare sample with Compat-Able Protein Assay Preparation Reagent Set (Cat. No. 23215). Finally, Thermo Scientific 660 nm Assay Kit (Cat. No. 22662) can also be used if protein extract is diluted two fold.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How do I process bacterial samples with EasyPep MS Sample Prep kits?

Add 1-2 µL of 50 mg/mL Lysozyme Solution (Cat. No. 90082) to 200 µL of the EasyPep lysis buffer provided in the kit and proceed with the sample prep. Add 200 µL of the prepared lysis solution to 5 X 10E8 bacterial cells (E. coli cells) (OD600 equal to 1, corresponding to 1 X 10E8 CFUs). Lyse by pipetting the sample repeatedly and centrifuge at 16,000 x g for 5 mins. Alternatively, E. coli cells can be lysed in lysis buffer using bead beating or sonication instead of lysozyme. After lysis, dilute samples to 1 mg/mL using lysis buffer, proceed with the EasyPep protocol for reduction, alkylation, digestion, and clean up as described in the product manual.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can you explain how the B-PER Bacterial Protein Extraction Reagent lyses cells?

The B-PER Reagent solution contains a proprietary, mild, non-ionic detergent in 20 mM Tris-HCl, pH 7.5. It effectively disrupts cells and solubilizes native or recombinant proteins without denaturation. The reagent creates holes in the cell membrane that will leak out cytosolic proteins. The sample may become very viscous when the bacterial chromosome is released. We recommend adding DNAse I (Cat. No. 90083) to the reagent to reduce viscosity. For better lysis efficiency and if there are inclusion bodies, we recommend adding Lysozyme (Cat. No. 90082) to the reagent. Alternatively, you may purchase the B-PER Bacterial Protein Extraction Reagent with Enzymes Kit (Cat. No. 90078 or 90079) that includes the B-PER Bacterial Protein Extraction Reagent, DNase I, and Lysozyme.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.