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View additional product information for Rapid Equilibrium Dialysis (RED) Inserts and Plates - FAQs (90088, 90087, 90112, 91012, 90004, 90006, 90005, 99006, 89809, 90085, 89812, 89811, 90007, 89810, 90006B)
16 product FAQs found
A final concentration of 1% DMSO is acceptable and will not affect the study. While DMF has not been tested, it is expected that DMF should be similar to DMSO in terms of use.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
You can add up to a final concentration of 0.5 M salt.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Each sample requires two chambers for equilibrium dialysis to take place (96/2 = 48); one chamber for the plasma sample, the other for buffer.
We have not tested the units with cells or viral particles. Customers would have to design and optimize their experiments to use the RED device with this type of sample.
Plasma samples from human, mouse, and rat are typically tested for binding of ligands with the rapid equilibrium dialysis (RED) device. For toxicology studies, a monkey sample is also tested. Typically, pooled plasma samples purchased from commercial vendors are used, although researchers could test the differences in plasma from various physiological states using the RED devices.
The container for the device is Teflon (PTFE), the insert is made of high-density polyethylene (HDPE), and they are highly hydrophobic. The regenerated cellulose membrane is a standard material for commercial dialysis devices. A recovery study consistently shows 85% recovery of high and low protein binding compounds. This result is indicative of minimal nonspecific binding, since the recovery between the membrane side and the PTFE/HDPE housing side showed very small difference.
In most cases, 4 hours is sufficient to reach equilibrium. This is due to the high surface area-to-volume ratio for the membrane (7.4:1), but can vary with different compounds.
No. They are manufactured in an ISO environment. The Teflon plate is also ISO-certified.
No. The units do not undergo a sterilization procedure nor are they tested for endotoxin content.
Yes. The Teflon Base Plate has the standard 96-well footprint. It can be used with any system compatible with standardized 96-well ELISA plates with 9 by 9 mm well spacing. The single-use format for the RED Device is especially convenient for labs using radioactive materials because the plate can easily be disposed of to minimize contamination and the need for cleaning.
We offer 8K and 12K MWCOs.
The membrane used is regenerated cellulose with a low glycerol content (acts as a humectant).
Please use our selection table (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-mass-spectrometry-analysis/plasma-protein-binding-equilibrium-dialysis.html) to choose the right plasma or tissue-to-plasma protein binding product for your experiment.
Equilibrium dialysis uses size-defined membranes to separate the free molecules (i.e., those molecules not bound to plasma proteins at equilibrium state). This type of dialysis mimics an in vivo environment. We offer our RED (rapid equilibrium dialysis) and competition RED systems to conduct plasma or tissue-to-plasma protein-binding studies.
Please view our selection table to choose the right dialysis device for your experiment - https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/protein-dialysis-desalting-concentration/dialysis-products.html.
Dialysis is the separation of small and large molecules in a solution by selective diffusion through a semi-permeable membrane. It is generally used for larger sample volumes, and can take hours to overnight for complete dialysis.