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查看更多产品信息 GeneChip™ Human Mitochondrial Resequencing Array 2.0 - FAQs (900886)
4 个常见问题解答
The Human Mitochondrial Resequencing Array Library files can be installed as follows:
- Go to the product page: https://www.thermofisher.com/order/catalog/product/900886?SID=srch-srp-900886
- Click on Technical Documents
- Download the library files to the local computer
- Open the AGCC Library file installer
- In AGCC Library file installer choose: Import parameters from a GCOS library file package CD or folder
- Click Next
- In the Select CD drive or folder location of source files to import: click on Browse and navigate to location of Library file that was just downloaded. Do not change the Save location
- Click Next.
- The library files available to install will be listed.
- Choose the Mitochip_2
- Click Start.
- The software will prompt that the files were successfully imported.
- Close the Library file importer. The Library files are now installed.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
We recommend the GeneChip Resequencing Assay Kit (Cat. No. 900447), which is sufficient for 30 reactions. gDNA input is dependent on the array format. The GeneChip Resequencing Assay Kit is 169 format, therefore gDNA input is 5 µL at 5 ng/µL
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
The GeneChip Human Mitochondrial Resequencing Array 2.0 interrogates the entire 16 kb mitochondrial genome. Sequences were obtained from the MITOMAP database Revised Cambridge Reference Sequence (RCRS). The array also contains additional tiling for the most common variants in the HVI and HVII regions. There are 8 unique 25-mer probes per base position (4 oligonucleotide probes per strand).
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
For array hybridization, washing, staining and scanning, the following instrumentation is required:
-GeneChip Scanner 3000 7G
-Fluidics Station 450
-Hybridization Oven 640 or Hybridization Oven 645
Data acquisition and analysis of Mouse 430 2.0 Arrays requires the following software:
-GeneChip Command Console Software
-Expression Console Software
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.