iodoTMTzero™ Label Reagent, 5 x 0.2 mg, 5 x 0.2 mg - FAQs

查看更多产品信息 Thermo Scientific™ iodoTMT Isobaric Label Reagents and Kits - FAQs (90101, 90100, 90102)

2 个常见问题解答

What is the difference between IodoTMT tags and Iodoacetamido-LC-Phosphonic Acid?

Both IodoTMT tags and Iodoacetamido-LC-Phosphonic Acid react with sulfhydryl groups. Iodo TMT tags allow the quantitation of proteins with cysteine amino acids and the oxidative state of sulfhydryl groups, while Iodoacetamido-LC-Phosphonic Acid allow the detection and enrichment of those specific proteins. This is a great tool for mass spectrometry (MS) analysis, and High-Select Fe-NTA Magnetic Phosphopeptide Enrichment Kit (Cat. No. A52283), can be used for the enrichment step.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Why can I only process 48 samples on a 96-well plate with the rapid equilibrium dialysis (RED) system?

Each sample requires two chambers for equilibrium dialysis to take place (96/2 = 48); one chamber for the plasma sample, the other for buffer.