GeneChip™ Mouse Gene 1.0 ST Array
GeneChip™ Mouse Gene 1.0 ST Array
Applied Biosystems™

GeneChip™ Mouse Gene 1.0 ST Array

GeneChip™ Mouse Gene 1.0 ST Array 是一个全转录本阵列，包括测量信使 (mRNA) 和长链基因间非编码 RNA了解更多信息
Have Questions?
更改视图buttonViewtableView
货号阵列数量
90117130 阵列
9011682 阵列
9011696 阵列
货号 901171
价格（CNY)
-
阵列数量:
30 阵列
GeneChip™ Mouse Gene 1.0 ST Array 是一个全转录本阵列，包括测量信使 (mRNA) 和长链基因间非编码 RNA 转录本 (lincRNA) 的探针。这种全转录本阵列设计提供 mRNA 的完整表达谱以及影响 mRNA 表达谱的中间 lincRNA 转录本。

全面的设计
过去 20 年的研究主要关注编码蛋白的 mRNA 及其在细胞过程（如疾病发展）中的作用。近期，研究人员已经在小鼠基因组中发现了 2,000 多个转录本 （>200 个碱基），几乎没有/没有蛋白质编码潜能。到目前为止，这些非编码 RNA 中仅有一小部分具备功能注释。然而，有充分证据表明，lincRNA 的差异表达在疾病的发生和进展中起着重要作用，并且这些分子的异常表达也与癌症相关。转录组谱分析的最新进展提供了在各种细胞功能中 lincRNA 关联的证据：

•mRNA 转录调控
•mRNA 转录后修饰的调控
•转录因子结合的活化/封闭
•转录因子的激活和转运
•与辅助蛋白相互作用
•将蛋白质复合物引导至基因组位置

关键优势
•覆盖范围广有助于发现感兴趣的生物学信息
   - >28,000个编码转录本
   - >7,000个非编码（包括˜2,000 个）长链基因间非编码转录本
•用探针检测选择性剪接事件/转录物变体，设计的探针具有较大的外显子覆盖率
•重现性：批次内相关系数 = 0.99

内容概况
自设计出 GeneChip 小鼠基因 1.0 ST 阵列以来，对于小鼠基因组结构和功能的理解已有了很大提升。这种认知的增加包括识别大量长链基因间 lincRNA，这些均由研究界发现。为了向研究界提供一种可以测定令人兴奋的 RNA 转录本差异表达的工具，我们设计了 GeneChip 小鼠基因 2.0 ST 阵列（单独出售）。为了补充 RefSeq 中包含的 lincRNA 数据，我们使用了 lncRNA 数据库 (http://lncrnadb.cn/) 的序列和转录本数据。
仅供科研使用。不可用于诊断程序。
规格
产品线GeneChip™
数量30 arrays
类型小鼠基因 1.0 ST 阵列
数组转录组谱分析
产品规格阵列检测盒
阵列数量30 阵列
种属小鼠
Unit SizeEach

常见问题解答 (FAQ)

What is contained in the tab-delimited format of the GeneChip probe sequence download file?

The tab-delimited probe sequence file contains the following information:
-Probe Set Name
-Probe X: The X coordinate of the probe sequence on the GeneChip probe array.
-Probe Y: The Y coordinate of the probe sequence on the GeneChip probe array.
-Probe Interrogation Position: The base position on the consensus/exemplar sequence where the central base of the probe aligns, which is the 13th base of a 25mer probe.
-Probe Sequence: The 25-base perfect match sequence.
-Target Strandedness: The sense/antisense orientation of the target sequence that can hybridize with the probe sequence.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What is the NetAffx Analysis Center?

The NetAffx Analysis Center used to contain information and files regarding array content, probe sets, and functional annotations. However, the NetAffx Analysis Center has been retired. Much of the content provided by the NetAffx Analysis Center can now be accessed on the arrays’ thermofisher.com product pages or by contacting Technical Support (techsupport@thermofisher.com).

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Are pseudogene databases included in the design of expression arrays?

Pseudogene databases were not included in the design of expression arrays.

How long can I store labeled cDNA when working with expression microarrays?

Labeled material can be stored for 2 weeks at -20 degrees C.

Is the protocol same for Exon 1.0 St and Gene 1.0 St arrays?

The protocols for the Gene 1.0 ST and Exon 1.0 ST arrays are the same until the fragmentation and labeling steps.
Depending on the input amount, please refer to the GeneChip WT Pico Reagent Kit or the GeneChip WT PLUS Reagent Kit for the protocol:
- WT Pico: 50 - 500ng of total RNA
- WT PLUS: ≥ 100pg of total RNA (approx. 10 cells)
From the hybridization step onwards, the differences in protocol are due to the format of the arrays. The Exon array is a 49 format array, whereas the Gene 1.0 ST array is a 169 format array.
For the fluidics step the following scripts should be used depending upon which array you are using:
- Gene 1.0 St array: FS450_0001
- Exon 1.0 St array: FS450_0007

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

View all GeneChip™ Mouse Gene 1.0 ST Array FAQs