GeneChip™ miRNA 2.0 Array, 6 arrays - FAQs

查看更多产品信息 GeneChip™ miRNA 2.0 Array - FAQs (901753TS, 901755, 901754)

3 个常见问题解答

Where can I find the species information for your GeneChip miRNA arrays?

The species information can be found in the annotation file of the miRNA array of interest.
Please complete the following steps:
- Go to www.thermofisher.com and go to the product page of the array of interest.
- Scroll down to support files.
- Download the annotation files for the miRNA array of interest, and open in excel.
- In the file, there will be a column, species scientific name. This column will have all the species present on the array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

For GeneChip miRNA arrays, are External RNA Controls Consortium (ERCC) controls tiled on the array?

The ERCC controls are not tiled onto GeneChip miRNA arrays because they would not work with the miRNA array/assay system. The FlashTag Biotin HSR RNA Labeling Kits used to label miRNAs is optimized for shortRNAs. Even though longer mRNAs like the ERCC controls will be labeled, the HWS (hyb/wash/stain) conditions are not optimal and consequently, the longer mRNAs typically do not get detected.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Does having multiple copies of the same probe across species impact hybridization in miRNA arrays?

Due to certain miRNAs being conserved across species, there may be multiple copies of the same probe across the miRNA arrays. However, sample processing with the FlashTag Biotin HSR RNA Labeling Kits ensures that there is excess target compared to the probes on the array. Consequently, there is enough sample available to hybridize onto all the replicate probes.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.