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View additional product information for GeneChip™ Arabidopsis Gene 1.0 ST Array - FAQs (901916, 901915)
12 product FAQs found
Pseudogene databases were not included in the design of expression arrays.
Labeled material can be stored for 2 weeks at -20 degrees C.
The protocols for the Gene 1.0 ST and Exon 1.0 ST arrays are the same until the fragmentation and labeling steps.
Depending on the input amount, please refer to the GeneChip WT Pico Reagent Kit or the GeneChip WT PLUS Reagent Kit for the protocol:
- WT Pico: 50 - 500ng of total RNA
- WT PLUS: ≥ 100pg of total RNA (approx. 10 cells)
From the hybridization step onwards, the differences in protocol are due to the format of the arrays. The Exon array is a 49 format array, whereas the Gene 1.0 ST array is a 169 format array.
For the fluidics step the following scripts should be used depending upon which array you are using:
- Gene 1.0 St array: FS450_0001
- Exon 1.0 St array: FS450_0007
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
To install library files for the Gene 2.0 ST Array for Command Console, simply run the executable library file installer.
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Installing the library files for the Gene 1.0 ST Array for AGCC requires the use of the Affymetrix Library File Importer which converts GCOS library files into AGCC format, then imports the files to the Command Console library file folder. The Library File Importer can convert files from the local GCOS installation, from a GCOS server on a remote Windows Server, or from a GCOS library file package on a CD or disk drive.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
The Gene 2.0 ST Array is a 100 format array.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
The GeneChip Gene 1.0 ST Array is an 81/4 format. Please note that 81/4 format is to be treated equivalent to 100 format arrays.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
FS450_00002 should be used when processing Gene 2.0 ST Array. Up-to-date fluidics scripts can be obtained from the website.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
FS450_00007 should be used when processing Gene 1.0 ST Array. Up-to-date fluidics scripts can be obtained from the website.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
GeneChip Operating Software (GCOS) or GeneChip Command Console Software (AGCC) can be used to process Gene 1.0 ST Arrays through fluidics and scanning. Gene 2.0 ST Arrays utilize an updated installer package that is not compatible with GCOS. Gene 2.0 ST Arrays require AGCC for fluidics and scanning. Affymetrix Expression Console Software is required to perform QC analysis of q4Gene 1.0 ST and Gene 2.0 ST Array data. Expression Console Software can be downloaded free from
the website.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
Using the Ambion WT Expression kit, a minimum of 50 ng of starting total RNA is required. No ribosomal RNA reduction of the starting material is required.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
The WT Plus kit or the WT Pico kit is recommended for preparing samples for use with the Gene 1.0 ST Array and the Gene 2.0 ST Array.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.