GeneChip™ WT PLUS Reagent Kit
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Applied Biosystems™

GeneChip™ WT PLUS Reagent Kit

GeneChip WT 阵列芯片是一种经过充分验证的基因转录本谱分析技术,可用于表达研究和生物标记物发现。GeneChip WT PLUS Reagent Kit(WT PLUS了解更多信息
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货号数量
90228010 Reactions
货号 902280
价格(CNY)
-
数量:
10 Reactions
GeneChip WT 阵列芯片是一种经过充分验证的基因转录本谱分析技术,可用于表达研究和生物标记物发现。GeneChip WT PLUS Reagent Kit(WT PLUS 试剂盒),具有高灵敏度和特异性,可进行稳健的 WT 分析,可在基因和外显子水平阐明您样品中的转录标志。

WT PLUS 试剂盒使用逆转录启动法,可生成每个 RNA 转录本的全长(包括多聚 A 尾和非多聚 A 尾 mRNA),以便完成转录组的完全无偏倚覆盖。该试剂盒可以对正义 DNA 靶标进行扩增和生物素化,避免由于反义链干扰而出现特异性损失。

该试剂盒包括扩增模块(与 GeneChip WT 末端标记偶联)和对照试剂盒(用于从 50 ng 到 500 ng 的总 RNA 中制备杂交靶标)。

特点和优势:
• 一个完整的靶标制备试剂盒具有经验证的灵敏度和特异性(由 WT 阵列的优化设计实现)
• 仅需输入少量样品 (50 ng)。该试剂盒适用于多种样品类型,包括新鲜冷冻组织、细胞系和全血
• 简化的工作流程,无须去除核糖体 RNA 或者进行血液样品的球蛋白还原
• 可将 WT 阵列与 WT 试剂共同使用,节约更多成本
• 便捷的单源订购和支持

相关链接
GeneChip HT WT PLUS Reagent Kit
Biomek FXP Target Prep Express 用 HT WT PLUS 耗材
仅供科研使用。不可用于诊断程序。
规格
适用于(应用)转录组谱分析
产品线GeneChip™
数量10 Reactions
试剂类型样品制备试剂
类型WT PLUS 试剂盒
Unit SizeEach

常见问题解答 (FAQ)

What reagent kit should I use with my array?

Please refer to the Microarray Reagent Guide for Arrays and Expression Kits to match the correct reagents your array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Where can I download the control sequences for expression arrays?

Sequences of all our controls can be downloaded from the sequence file that is available on product pages on thermofisher.com.

For example, here is the link to the product page for Clariom D Assay, human: https://www.thermofisher.com/order/catalog/product/902923
Go to the "Software & Data Analysis" section and under "Support Files", click on "Sequence Files: Clariom D Array, human Probe Sequences, tabular format", which you can open in Excel.

Note: If the control sequence file is not available, please contact techsupport@thermofisher.com.

Are pseudogene databases included in the design of expression arrays?

Pseudogene databases were not included in the design of expression arrays.

What is the shelf life of the Poly-A RNA Control's first dilution during processing of expression microarrays?

The first dilution of the Poly-A RNA Control can be stored at -20 degrees C for 6 weeks (with up to 4 freeze/thaws).

How long can I store labeled cDNA when working with expression microarrays?

Labeled material can be stored for 2 weeks at -20 degrees C.