Is the hybridization mix for cartridges the same as the hybridization mix for PEG arrays (array plates)?
The hybridization mix for PEG arrays is different from the hybridization mix for cartridges. The concentrations of the hybridization mix are different and in addition, DMSO is not added into the hybridization mix for PEG arrays because it can affect the glue that holds the PEG to the plate.
Where can I find the species information for your GeneChip miRNA arrays?
The species information can be found in the annotation file of the miRNA array of interest.
Please complete the following steps:
- Go to www.thermofisher.com and go to the product page of the array of interest.
- Scroll down to support files.
- Download the annotation files for the miRNA array of interest, and open in excel.
- In the file, there will be a column, species scientific name. This column will have all the species present on the array.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
How are sno/sca and hairpin probe sets selected and how does that impact my signal summarization?
Probes for snoRNA, scaRNA, and hairpins are selected to maximize probe response to target concentration in the sample while minimizing cross-hybridization to other potential targets in the sample. In order to minimize cross-hybridization, potential targets are inferred from the landscape of known sequences from the input dataset and used in a filtering process called pruning which penalizes probe candidates that may cross-hybridize to unwanted targets. As the landscape of known sequences improves, we can improve our pruning set to avoid probe candidates previously thought to be unique. An improved pruning set will reduce the chances a probe will cross-hybridize to unwanted target, improving the probe set representation of the intended target. As a result of the improved probe selection, few probe sets are required to represent the same number of sequences.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
Can I combine the CEL files from GeneChip miRNA Arrays, miRNA Array Strips, and miRNA Array Plates into the same experiment?
No, the CEL files from GeneChip miRNA Array, Thermo Fisher Scientific miRNA Array Strip, and miRNA Array Plates are not compatible.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
What is the difference between GeneChip miRNA Array, miRNA Array Strip, and miRNA Array Plate?
The only difference is the form factor of the array. The arrays have the exact same design and contain the same number of probes and probe sets.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.