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View additional product information for GeneChip™ miRNA 4.1 Array Plate - FAQs (902409, 902408TS, 902410)
14 product FAQs found
The hybridization mix for PEG arrays is different from the hybridization mix for cartridges. The concentrations of the hybridization mix are different and in addition, DMSO is not added into the hybridization mix for PEG arrays because it can affect the glue that holds the PEG to the plate.
The species information can be found in the annotation file of the miRNA array of interest.
Please complete the following steps:
- Go to www.thermofisher.com and go to the product page of the array of interest.
- Scroll down to support files.
- Download the annotation files for the miRNA array of interest, and open in excel.
- In the file, there will be a column, species scientific name. This column will have all the species present on the array.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
Probes for snoRNA, scaRNA, and hairpins are selected to maximize probe response to target concentration in the sample while minimizing cross-hybridization to other potential targets in the sample. In order to minimize cross-hybridization, potential targets are inferred from the landscape of known sequences from the input dataset and used in a filtering process called pruning which penalizes probe candidates that may cross-hybridize to unwanted targets. As the landscape of known sequences improves, we can improve our pruning set to avoid probe candidates previously thought to be unique. An improved pruning set will reduce the chances a probe will cross-hybridize to unwanted target, improving the probe set representation of the intended target. As a result of the improved probe selection, few probe sets are required to represent the same number of sequences.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
No, the CEL files from GeneChip miRNA Array, Thermo Fisher Scientific miRNA Array Strip, and miRNA Array Plates are not compatible.
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The only difference is the form factor of the array. The arrays have the exact same design and contain the same number of probes and probe sets.
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Probes on the array detect sense target.
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GeneChip miRNA Array 130 µL of hybridization cocktail
Thermo Fisher Scientific miRNA Array Plates 120 µL of hybridization cocktail
Thermo Fisher Scientific miRNA Array Strips 120 µL of hybridization cocktail
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Minimum is 130 ng; maximum is 1,000 ng total RNA.
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The arrays have the exact same design and contain the same number of probes and probe sets.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
You can get the list by downloading the annotation files that will be available on the website.
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The array plates should be stored at 2-8 degrees C.
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This product has 12 months of shelf life with a minimum shelf life of 3 months. Minimum shelf life is defined as the minimum amount of time from date of order shipment by ThermoFisher Scientific to array expiration date. If users receive products with shorter than expected minimum shelf life, we will replace the products at no cost to the user-subject to terms and conditions.
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The CEL files generated from this array is compatible with Transcriptome Analysis Console and can be used for normalization, signal summarization and differential expression analysis of your experimental data.
For analysis in Transcriptome Analysis Console Software, array type specific configuration and annotation files are required. Use the “Download Array Type Files” button in the Preferences tab to download and install array type specific library files.
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We only support use of the Affymetrix FlashTag Biotin HSR RNA Labeling Kit for preparing targets to be applied to GeneChip miRNA Array .
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