Search

  • 联系我们
  • 货号直购

GeneChip™ HT 3' IVT PLUS Reagent Kit - FAQs

查看更多产品信息 GeneChip™ HT 3' IVT PLUS Reagent Kit - FAQs (902417)

9 个常见问题解答

What reagent kit should I use with my array?

Please refer to the Microarray Reagent Guide for Arrays and Expression Kits to match the correct reagents your array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Where can I download the control sequences for expression arrays?

Sequences of all our controls can be downloaded from the sequence file that is available on product pages on thermofisher.com.

For example, here is the link to the product page for Clariom D Assay, human: https://www.thermofisher.com/order/catalog/product/902923
Go to the "Software & Data Analysis" section and under "Support Files", click on "Sequence Files: Clariom D Array, human Probe Sequences, tabular format", which you can open in Excel.

Note: If the control sequence file is not available, please contact techsupport@thermofisher.com.

Are pseudogene databases included in the design of expression arrays?

Pseudogene databases were not included in the design of expression arrays.

How long can I store labeled cDNA when working with expression microarrays?

Labeled material can be stored for 2 weeks at -20 degrees C.

What is the shelf life for expression microarrays?

Typical shelf life for expression arrays are as follows:
- All ≥ 8 µm commercial expression cartridges are stable up to 24 months.
- All ≥ 8 µm commercial resequencing cartridges are stable up to 18 months.
- All ≥ 8 µm commercial expression plates are stable up to 18 months.

Please note that this excludes products such as Exon or Gene array as they are 5 µm cartridge and plates.

Is there a recommended RIN (RNA integrity number), based on Bioanalyzer, for RNA quality for IVT and WT assays?

For IVT assays, the RIN should be 6 or higher. For WT assays, there are no recommendations based on assay priming.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How many freeze/thaw cycles have been validated for use with the GeneChip HT 3' IVT PLUS assay?

We guarantee up to three freeze/thaws.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What starting material is needed for the GeneChip assay?

A total of 100 ng of total RNA per sample is the recommended starting quantity if following the standard protocol. A total of 50 ng can be used at a minimum.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Does genomic DNA contamination in the sample interfere with the results, and how do I monitor the degree of its effect?

By titrating genomic DNA back into the total RNA samples and monitoring the deterioration of the array data, it was determined during development of the assay that a moderate amount of genomic DNA contamination will only have minimum effect on the array results. Therefore, routine RNA isolation techniques coupled with DNase treatment should yield sufficiently high-quality sample for analysis on the exon arrays.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.