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查看更多产品信息 GeneChip™ Mouse Genome 430 2.0 Assay - FAQs (902490, 902491)
7 个常见问题解答
Please refer to the Microarray Reagent Guide for Arrays and Expression Kits to match the correct reagents your array.
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The gamma parameters defaults for these arrays were set to deliver specificity consistent with our other expression products. (The changes in the default gamma values apply to 11 micron arrays). These parameters are user definable.
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About 12 minutes per Mouse 430 2.0 Array, comparable to scan time for a set of Mouse 430A and Mouse 430B Arrays.
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A single Mouse 430 2.0 array generates approximately 130MB of data.
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Please contact Technical Support to obtain the high-resolution scanning patch.
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Below are a list of circumstances and clarification on if the high-resolution scanning patch needs to be applied. Keep in mind that the high-resolution scanning patch must only be applied on top of GCOS, not AGCC software.
a. GeneChip Scanner 3000 (serial numbers 502xxxxx): The instrument service engineer has applied the high-resolution scanning patch to the computer that is controlling the GeneChip Scanner 3000 at installation, but not to the workstations or servers (see c and d below).
b. GeneChip Scanner 3000 (serial numbers 501xxxxx): The instrument service engineer will apply the high-resolution scanning patch to the computer that is controlling the GeneChip Scanner 3000 at the time of the upgrade, but not to the workstations or servers (see c and d below).
c. Analysis workstations and clients: You must apply the high-resolution scanning patch.
d. Server upgrade: Your database administrator or an Affymetrix Software Support Engineer needs to apply the high-resolution scanning patch in coordination with the upgrade of server to GCOS.
e. Installation of library files post scanner installation or upgrade: The high-resolution scanning patch must be reapplied following the installation of library files for arrays that precede the Mouse 430 2.0 Arrays. The Mouse 430 2.0 and library files are high-resolution-scanning ready. The error message "cannot read the parameters for probe array type from the database" appears when one tries to scan an array on a GeneChip Scanner 3000 high-resolution scanner but the high-resolution scanning patch has not been applied.
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a. GeneChip Scanner 3000s that were shipped after September 15, 2003 with serial number 502xxxxx are enabled for high-resolution scanning.
b. GeneChip Scanner 3000s that were shipped prior to September 15, 2003 with serial number 501xxxxx that have had the GeneChip Scanner 3000 High-Resolution Update installed by an instrument service engineer are enabled for high-resolution scanning. A sticker is applied to the rear of the scanner to indicate that the upgrade has been performed
c. Contact 888-DNA-CHIP or your local Affymetrix technical support to inquire about a scanner upgrade.
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