GeneChip™ 3' IVT Pico Kit, 30 Reactions - FAQs

View additional product information for GeneChip™ 3' IVT Pico Kit - FAQs (902790, 902789)

9 product FAQs found

What reagent kit should I use with my array?

Please refer to the Microarray Reagent Guide for Arrays and Expression Kits to match the correct reagents your array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Where can I download the control sequences for expression arrays?

Sequences of all our controls can be downloaded from the sequence file that is available on product pages on thermofisher.com.

For example, here is the link to the product page for Clariom D Assay, human: https://www.thermofisher.com/order/catalog/product/902923
Go to the "Software & Data Analysis" section and under "Support Files", click on "Sequence Files: Clariom D Array, human Probe Sequences, tabular format", which you can open in Excel.

Note: If the control sequence file is not available, please contact techsupport@thermofisher.com.

Are pseudogene databases included in the design of expression arrays?

Pseudogene databases were not included in the design of expression arrays.

What is the shelf life of the Poly-A RNA Control's first dilution during processing of expression microarrays?

The first dilution of the Poly-A RNA Control can be stored at -20 degrees C for 6 weeks (with up to 4 freeze/thaws).

How long can I store labeled cDNA when working with expression microarrays?

Labeled material can be stored for 2 weeks at -20 degrees C.

What is the shelf life for expression microarrays?

Typical shelf life for expression arrays are as follows:
- All ≥ 8 µm commercial expression cartridges are stable up to 24 months.
- All ≥ 8 µm commercial resequencing cartridges are stable up to 18 months.
- All ≥ 8 µm commercial expression plates are stable up to 18 months.

Please note that this excludes products such as Exon or Gene array as they are 5 µm cartridge and plates.

Is there a recommended RIN (RNA integrity number), based on Bioanalyzer, for RNA quality for IVT and WT assays?

For IVT assays, the RIN should be 6 or higher. For WT assays, there are no recommendations based on assay priming.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What are the available assay options for running the Clariom S array?

Depending on the sample type, input RNA amount, and required instruments, there are several cartridge and array plate options.
- Cartridge with GeneChip Pico Kit for 100pg–50 ng of total RNA from whole blood, cultured cells, and fresh/fresh-frozen or FFPE tissues.
- Cartridge with GeneChip WT Plus Reagent Kit for 50–500 ng of total RNa isolated from whole blood, cultured cells, and fresh/fresh-frozen tissues.
- 24 or 96-array plate with GeneChip Pico Kit for 100pg–50 ng of total RNA from whole blood, cultured cells, and fresh/fresh-frozen or FFPE tissues, for analysis on the GeneTitan MC instrument.
- 24 or 96-array plate with GeneChip WT Plus Reagent Kit for 50–500 ng of total RNA isolated from whole blood, cultured cells, and fresh/fresh-frozen tissues, for analysis on the GeneTitan MC instrument.
Additional information about the Clariom S options offered can be found in the Data Sheets: Clariom S Solutions document on the Thermo Fisher website.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Why do I need to use the Control Oligo B2 with my assay?

Control Oligo B2 (3 nM) is a pre-labeled DNA spike-in control required for hybridization to the control probes on the array utilized for grid alignment by AGCC. A failure to include Control Oligo B2 in the hybridization cocktail will result in the inability of the software to apply a grid over the scanned image, leading to the unrecoverable loss of sample data from the array. The absence of the Control Oligo B2 also voids the customer's ability to submit an array replacement request.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.