Axiom™ Microbiome, 96 array plate - FAQs

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9 product FAQs found

Is the hybridization mix for cartridges the same as the hybridization mix for PEG arrays (array plates)?

The hybridization mix for PEG arrays is different from the hybridization mix for cartridges. The concentrations of the hybridization mix are different and in addition, DMSO is not added into the hybridization mix for PEG arrays because it can affect the glue that holds the PEG to the plate.

What is the starting amount of DNA required for Axiom arrays?

All Axiom arrays (except for Axiom Genome-Wide Pan-African Array Set) require a total of 200 ng DNA. The Axiom Genome-Wide Pan-African Array Set requires a total of 300 ng DNA, or 100 ng DNA per array (there are 3 arrays).

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What are the sample requirements for Axiom array processing at the Thermo Fisher Scientific Microarray Research Services Laboratory (MRSL)?

For information regarding our in-house MRSL requirements, please contact us via phone at 888-362-2447 (in North America) or via email at BioinformaticsServices@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How many and what species are not detected by the Axiom Microbiome Array?

81 species are not detectable on Axiom Microbiome Array. For a complete list of species not detected, please contact the Technical Support Team.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How many and what species can be detected by the Axiom Microbiome Array?

12,513 species can be detected on Axiom Microbiome Array. These are species from five microbial domains; archaea, bacteria, fungi and protozoa. For a complete list of species, please contact the Technical Support Team.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What extraction kit should be used for the fecal samples for the Axiom Microbiome Array (Cat. Nos. 902904, 902903)?

Any extraction and purification kit that meets the starting gDNA requirements is acceptable. However, methods that require boiling or strong denaturants should not be used, as the DNA will be rendered single-stranded.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What sources of genomic DNA (gDNA) have been tested to use with the Axiom Microbiome Array (Cat. Nos. 902904, 902903)?

gDNA extracted from stool samples has been tested for use with the Axiom Microbiome Array. DNA derived from FFPE (formalin-fixed paraffin-embedded) blocks should not be used with this assay.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What was the criteria for selecting covered species on the Axiom Microbiome Array?

The species included on the array represent all known microbial sequences available in the NCBI database as of October 2014 for which probes could be designed. Any sequences deposited after October 2014 are not represented on the array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What is the required input for the Axiom Microbiome Array (Cat. Nos. 902904, 902903)?

50 ng of gDNA (20 µL at 2.5 ng/µL) is optimal. The gDNA must also be double-stranded, of high purity (OD260/OD280 ratio of 1.8-2.0 and OD260/OD230 ratio greater than 1.5), and not degraded (90% of gDNA greater than 10 kb).

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.