Clariom™ S Assay HT, human, 30 reactions - FAQs

View additional product information for Clariom™ S Assay HT, human - FAQs (902969, 902970)

8 product FAQs found

What reagent kit should I use with my array?

Please refer to the Microarray Reagent Guide for Arrays and Expression Kits to match the correct reagents your array.

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How do I install Expression Console Software and TAC Software library files for human, mouse, or rat transcriptome assays?

Analysis library files and annotation files can be downloaded from within the TAC Software. Please refer to the respective user manuals for detailed instructions.

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What software is available for analyzing the results from my human, mouse, or rat transcriptome assays?

Probe cell intensity data (CEL) from human, mouse, or rat transcriptome assays are analyzed in Transcriptome Analysis Console(TAC) Software. The application uses a version of RMA analysis to create CHP files. Transcriptome Analysis  Console Software can be downloaded at no charge from the website. Further statistical analysis may be performed in Affymetrix' Transcriptome Analysis Console (TAC) Software to obtain a list of differentially expressed genes and alternative splicing events. TAC Software also provides the visualization of genes, exons, junctions, transcript isoforms, pathways, and mRNA-miRNA regulatory networks.

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How do I install AGCC library files for the human, mouse, or rat transcriptome arrays?

To install library files for the human, mouse, or rat transcriptome arrays for AGCC, simply run the executable library file installer.

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What format are the human, mouse, or rat transcriptome arrays?

Human, mouse, or rat transcriptome arrays are 49-format.

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Which fluidics protocol should I use when processing human, mouse, or rat transcriptome assays?

FS450_0001 should be used when processing human, mouse, or rat transcriptome assays. Up-to-date fluidics scripts can be obtained from the website.

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Can I analyze the results from my human, mouse, or rat transcriptome assays using GeneChip Operating Software (GCOS) or GeneChip Command Console Software (AGCC)?

Human, mouse, or rat transcriptome assays utilize an updated installer package that is not compatible with GCOS. Human, mouse, or rat transcriptome assays require AGCC for fluidics and scanning. The Expression Console Software is required to perform QC analysis of the human, mouse, or rat transcriptome array data. Expression Console Software can be downloaded at no charge from the website.

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Which protocol should I use to prepare my sample for hybridization to GeneChip human, mouse, or rat transcriptome assays?

Affymetrix human, mouse, and rat transcriptome assays are available in three formats based on sample type and amount of input RNA:

Human, mouse, and rat transcriptome Pico assays compatible with 100-2,000 pg of RNA isolated from fresh, frozen, blood, or FFPE samples
Human, mouse, and rat transcriptome FFPE assays compatible with 20-200 ng of RNA isolated from FFPE samples
Human, mouse, and rat transcriptome assays compatible with 50-500 ng of RNA isolated from fresh, frozen, or blood samples

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