The Applied Biosystems CytoScan HT-CMA 96F Assay Kit enables the detection of chromosomal abnormalities such as deletions, duplications, and uniparental disomy (UPD), and does so in a high-throughput, automated format. The assay is designed for use with genomic DNA from blood or amniotic fluid samples. Please see the CytoScan HT-CMA Assay Training Kit for arrays, reagents, and sample plates sufficient for the FAS led training week as well as the following proficiency run.

Features of the CytoScan HT-CMA 96F Assay Kit include:
• Consolidated testing with a dual CNV and SNV design
• Increased sample throughput with a fully automated assay protocol
• Fast and easy high-resolution genome-wide copy number analysis with the free Chromosome Analysis Suite software

High-resolution genome-wide copy number and SNV analysis in one
The CytoScan HT-CMA assay enables cytogenetic researchers to detect and analyze relevant chromosomal aberrations with confidence. The assay provides high-resolution coverage of OMIM and DECIPHER genes, along with high-density SNP coverage for loss of heterozygosity (LOH) and UPD detection. The CytoScan HT-CMA assay also includes up to 178 relevant single nucleotide variants (SNV) and content for analysis of SMN1, which allows for consolidation of testing.

High-throughput array format and full automation
Bring consistency to your cytogenetic analysis and free up technician time with the fully automated CytoScan HT-CMA assay. Preparation of gDNA for hybridization onto the arrays is automated on the NIMBUS Target Preparation Instrument, with automated array processing occurring on the GeneTitan MC Instrument.

Chromosome Analysis Suite-designed for cytogenetic applications
The popular Chromosome Analysis Suite (ChAS) software is available free of charge as part of the CytoScan HT-CMA assay. ChAS software is tailored to cytogenetic research analysis and reporting with:
• Streamlined analysis and reporting workflow
• Ability to apply customized filters to analyze the genome at different levels of resolution
• Options to create, modify, and upload annotation files and flag regions for focused analysis
• A database for storage, query, and visualization of samples
• Direct access to external databases such as NCBI, UCSC Genome Browser, Ensembl, and OMIM
• Trio consistency checking for parental studies

The new standard in high-throughput cytogenetic analysis
The combined high resolution DNA copy number data and the ability to screen a panel of SNVs on a single array makes the CytoScan HT-CMA assay the new standard for high throughput cytogenetics analysis.