HS BAC CLONE - CITB LIB - FAQs

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3 个常见问题解答

对我感兴趣的基因，使用基因组BAC克隆代替cDNA克隆的原因是什么？

BAC克隆为包含很大插入片段（>100 kb）的基因组克隆，可能包含几个基因，而cDNA克隆只包含一个开放读码框基因。BAC克隆适用于启动子及内含子／外显子分析，染色体定位及转基因小鼠制备。

What are some reasons why I should pick a genomic BAC clone for my gene instead of a cDNA clone?

BAC clones are genomic clones containing very large inserts (>100 kb) that may contain several genes, whereas cDNA clones only contain a single open reading frame of a gene. BAC clones are used for promoter and intron/exon analysis, chromosome localization, and making transgenic mice.

How do I find/order an individual BAC/PAC clone?

There are several internet sites that may be used to locate BAC/PAC clones. One of them is listed below:

- Go here.
- Select the species of interest from the “Select organism” box.
- Type the feature, location or gene name in the “Search in genome” box on the right.
- As an example, choose “Search in genome”, enter “ITGA5” as the “Feature name” and click the search icon.
- Click on “Tracks shown”.
- Find the Clone library of interest, click on “Genomic Clones” and then select the box for the library of interest: for example: RP23 (RPCI-23 Female (C57BL/6J) Mouse BAC Library) and click “Configure”.
- In the refreshed window, you will see a list of BAC/PAC clones containing the ITGA5 gene (for example: RP23-427P19 where the Clone ID is 427P19).
- Go to CloneRanger, select the “BAC Mouse RP23” Collection, type in the Clone ID (in this case, 427P19), and click “Submit” to see if we carry the BAC clone. If the order is being placed by phone, provide the Cat No. (RPCI23.C) and the Clone address (in this case, RP23-427P19).