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View additional product information for KingFisher™ Plastics for 96 deep-well format - FAQs (97003520, 97003540, 97003530, 97002570, 97002534B, 97002820B, 95040450BU, 95040450, 95040460, 95040460B, 97002534, 97002820, 95040450B, 97003500)
44 product FAQs found
不是，处理体积指的是24孔深孔板内可以装入的最大体积。这包括结合缓冲液的体积和磁珠体积，所以当扩大反应体积时，一定要确保总体积不超过5mL。
这种情况有时会发生，但是这不会影响产量，因为样本已经从磁珠上被洗脱下来了。
如果没有向磁棒套架插入磁棒套，则程序不会开始运行。
将软布或纸巾用温和的去垢剂、肥皂水或酒精润湿，然后擦拭磁力棒。
可以通过运行“维护 (Maintenance)”菜单之下的“更换磁头 (Change magnet)”程序实现，使用“上 (up)”和“下 (down)”按钮进行选择。
The KingFisher Flex磁体是使用非常稳定的材料制成的。磁场不会变弱。不过，极端的机械外力或加热可能会对磁体造成损坏。
注：务必使KingFisher Flex的磁棒头远离其他磁棒或其他磁体。磁体相互接触会引起严重损坏。不行，我们强烈推荐您使用MagMAX板或KingFisher板，因为它们是专为配合KingFisher Flex磁棒套同时使用而设计的，以达到最佳效果。其它制造商生产的板子可能与KingFisher Flex加热模块不兼容。它们还可能引起意料之外的问题，例如可能会由于不同的孔容积和板底高度而引起交叉污染。
带96深孔磁棒头的KingFisher Flex仪器和MagMAX Express-96仪器所需的耗材是相同的。列表如下：
- MagMAX Express-96 深孔板 (货号 4388476)
- MagMAX Express-96 标准板(货号4388475)
- MagMAX Express-96深孔磁棒套(货号4388487)
- MicroAmp 透明封口膜（可选）： (货号. 4306311)
对于带24深孔磁棒头的KingFisher Flex仪器，所需耗材列表如下：
- KingFisher Flex 24深孔板：(货号. 95040480)
- KingFisher Flex 24深孔磁棒套： (货号 97002610)
KingFisher Flex 磁珠处理仪有四种磁棒选择：
- 用于MagMAX Express-96深孔板的KingFisher Flex磁棒
- 用于KingFisher 24深孔板的KingFisher Flex磁棒
- 用于KingFisher 96孔板的KingFisher Flex磁棒
- 用于有裙边PCR板的KingFisher Flex磁棒
是的，所有MagMAX试剂盒都能用于KingFisher Flex 磁珠处理仪。
No, that specific catalog number (Cat. No 97002534) is not sterile and purity certified. However, there are other KingFisher plates and tip combs which are sterile and purity certified (see the following: Cat. No. 95040460, 97002820, 95040480 and 97002620).
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
The alternative is KingFisher Plastics for 96 deep‐well format (Cat. No. 97002534). This product is a 96 tip comb for deep‐well magnets that can be used on the KingFisher Flex and Presto instruments.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
Do not use the tip combs if the clips are bent or missing. Not having clips can cause the tip comb to drop from the holder during the run, which would cause the run to abort. If you received tip combs with bent or broken retaining clips, you can contact Technical Support at techsupport@thermofisher.com to request a free-of-charge replacement.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
If the tip does not load correctly onto a Thermo Scientific KingFisher magnetic head, we recommend that you try manually flexing the tips both lengthwise and widthwise to level the tip comb. A video of flexing the tip comb can be found here: https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/automated-purification-extraction/kingfisher-systems/resources.html. The video is under "KingFisher Flex" and titled "How to install the magnet head and check the tip comb." Flexing of the tip comb is demonstrated at timepoint 1:35. If that does not work, try a different box or lot of tips. This applies to all KingFisher instruments.
The KingFisher Flex instrument with the 24 Deep-Well magnetic head can process larger volumes for each sample (up to 5mL total volume) compared to the KingFisher Flex instrument with the 96 Deep-Well magnetic head.
Yes, the KingFisher Flex instrument 96-well magnetic head and the MagMAX Express-96 instrument magnetic head can use the same tip combs and KingFisher plates even though the magnetic heads are different.
Yes, we recommend using KingFisher 96 tip combs for deep-well format (Cat. No. 97002534 or 97002570).
No, KingFisher Plastics for 96-deep well format are not autoclavable. They won't melt, but they will get deformed.
The Kingfisher Plastics for 96 deep-well format are recommended for one-time use only, and we don't recommend reusing these products.
The KingFisher Plastics for 96 deep-well format are not CE marked.
Yes, depending on the kind of protocol and head that is used, the following consumables will have to be purchased:
- KingFisher 96 deep-well plate (Cat. No. 95040450)
- KingFisher 96 deep-well plate, sterile (Cat. No. 95040460)
- KingFisher 96 tip comb for deep-well (Cat. No. 97002534)
- KingFisher 96 deep-well tip comb and plate, sterile (Cat. No. 97002820)
- KingFisher 96 microplate (200 μL) (Cat. No. 97002540)
- KingFisher 96 tip comb (Cat. No. 97002524)
- MicroAmp Clear Adhesive Film (optional) (Cat. No. 4306311
No, the processing volume refers to the maximum volume that can be held in the 24 Deep Well plates. This incorporates the volume of binding buffers and beads, so when scaling up reaction volumes, it is important to make sure that the total volume does not exceed 5 mL.
No, once a protocol was stopped, it will restart from the beginning. The extraction procedure can either be finished manually by using a magnetic stand, or a modified version of the original KingFisher Flex script would have to be created that starts off at the point where the run was stopped.
Set up the protocol on the BindIt software. After the first 8 plates are done, the instrument will pause and instruct you to switch out the plates.
The transport locks are only necessary when relocating the instrument.
We strongly recommend that you maintain the specified volumes within the defined limits to avoid spillover and to help ensure the best performance at the most efficient level.
This happens sometimes but it will not affect the yield because the sample has been released from the particles.
If the starting material is too viscous, the magnetic rods will not be able to collect the particles. Dilute the sample and check that the sample is properly homogenized/lysed. Adding low amounts of detergent will improve the collection of magnetic particles as well. Quickly centrifuge the plate to sediment the particles to the bottom of the plate.
The protocol will not start without the tip combs inserted into the tip comb holder.
Wipe the magnetic rods with a soft cloth or tissue paper soaked in a mild detergent solution, soap solution, or alcohol.
The heating block warms up at the rate of about 10°C per minute, so it will take about 6 minutes.
Both deep-well plates and KingFisher 96 plates can be used during the same run. Therefore, it is possible to start the processing by using larger volumes (in a deep-well plate) and elute the purified sample to a smaller volume (in a KingFisher 96 or MagMAX 96 standard plate).
This can be done by running the Change magnet protocol under the Maintenance menu using the “up” and “down” cursor keys.
The KingFisher Flex magnets are made of material that is very stable. The magnetic field will not get weaker. However, extreme mechanical force or heating can cause damage to the magnets.
Caution: It is very important to keep the KingFisher Flex heads away from each other and from other magnets at all times. Clashing together of the magnets may cause serious damage to the magnets.
KingFisher Flex uses a USB port, whereas the MagMAX Express- 96 requires a RS-232 port.
No. Protocols cannot be transferred back from the instrument to the computer. Protocols need to be previously stored in the BindIt or KingFisher software to make edits.
No, manufactured protocols are locked for editing, but they can be renamed using the ‘save as' function and edited as a new version.
For nucleic acid extraction, we recommend using our MagMAX kits and MagJET kits. Other magnetic beads should work well as long as they are 0.5 to 10 µm in size. However, you will have to write your own KingFisher protocols using the Thermo Scientific BindIt software and validate/optimize the protocol on your own.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
Yes, this can be done by sample splitting. Set the protocol so that the magnet will collect samples from multiple wells before continuing to the next step. Be sure not to overload.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
Yes, reaction volumes can be scaled up, and all other volumes (i.e. wash volumes) will have to be increased by the same fold difference. It is generally not recommended to scale up reaction volumes more than 2-fold unless the plate's volume capacity will allow it.
No, we strongly recommend that you use the MagMAX plates or KingFisher plates since they were specifically designed to be used with KingFisher Flex tip combs to attain maximal performance. Plates from other manufacturers may not be compatible with the KingFisher Flex heating blocks. They may also cause unexpected problems, such as cross-contamination, due to the divergent well volume and bottom height of the plate.
The consumables for the KingFisher Flex instrument with the 96 Deep Well head are listed below:
- KingFisher 96 deep-well plate (Cat. No. 95040450)
- KingFisher 96 deep-well plate, sterile (Cat. No. 95040460)
- KingFisher 96 tip comb for deep-well (Cat. No. 97002534)
- KingFisher 96 deep-well tip comb and plate, sterile (Cat. No. 97002820)
- KingFisher 96 microplate (200 μL) (Cat. No. 97002540)
- KingFisher 96 tip comb (Cat. No. 97002524)
- MicroAmp Clear Adhesive Film (optional) (Cat. No. 4306311
For the KingFisher Flex instrument with the 24 Deep Well head, the following consumables are required:
- KingFisher 24 deep-well plate (Cat. No. 95040470)
- KingFisher 24 deep-well plate, sterile (Cat. No. 95040480)
- KingFisher 24 deep-well tip comb and plate (Cat. No. 97002610)
- KingFisher 24 deep-well tip comb and plate, sterile (Cat. No. 97002620)
There are 4 magnetic head options available for the KingFisher Flex Magnetic Particle Processor:
- KingFisher Flex head for MagMAX Express-96 Deep Well plate
- KingFisher Flex head for KingFisher 24 Deep Well plate
- KingFisher Flex head for KingFisher 96 plate
- KingFisher Flex head for PCR plate, skirted
Yes, all MagMAX kits can be used with the KingFisher Flex Magnetic Particle Processor.