Development and validation of a higher-throughput equilibrium dialysis assay for plasma protein binding.
Authorsvan Liempd S,Morrison D,Sysmans L,Nelis P,Mortishire-Smith R
JournalJournal of laboratory automation
PubMed ID21609686
We describe the practical aspects of developing a semiautomated, higher-throughput plasma protein binding (PPB) assay. The assay has a capacity of 32 PPB measurements per screen using triplicate incubations per measurement, and it is flexible with respect to the number of compounds and the number of plasma types used. The ... More
Development of a method to measure free and bound ropivacaine in human plasma using equilibrium dialysis and hydrophilic interaction chromatography coupled to high resolution mass spectrometry.
AuthorsAbbas M,Ahmad L,Shah Y,Gill M,Watson DG
JournalTalanta
PubMed ID24209310
The pharmacodynamics of absorption of local anaesthetics used during surgical procedures into tissues is governed by the amount of free drug in plasma. Toxicity may occur during continuous infusions if the levels of free drug become too high which may occur if the binding capacity of the α-1-acid glycoprotein present ... More