可通过流式细胞术来鉴定人体MSC,这些细胞通常具有CD73+,CD90+,CD105+,CD11b-和CD45-的标志物属性(Blood 109:4245 (2007))。人MSC表达Oct4,Sox2和Rex-1;这些mRNA均可通过RT-PCR技术进行鉴定(Arthritis Res Ther 9:204 (2007))。
什么是间充质干细胞(MSC)?
间充质干细胞(MSC)是最初从骨髓或脂肪组织中分离出来的多专能细胞,它们表现出进一步分化为骨,软骨和脂肪细胞的能力。在常规细胞培养条件下,从骨髓中分离出的MSC为纺锤形,并拥有贴附于未包被塑料培养皿上的独特能力(Arthritis Res Ther 9:204 (2007))。
How many times can I freeze/thaw the StemPro Chondrogenesis Supplement (Cat. No. A1006401) from the StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101)?
We do not recommend that you freeze/thaw the StemPro Chondrogenesis Supplement (Cat. No. A10064-01) from the StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101). Addition of supplement to the StemPro Osteocyte/Chondrocyte Differentiation Basal Medium (Cat. No. A1006901) is stable at 2-8 degrees C for up to 1 month, protected from light.