可通过流式细胞术来鉴定人体MSC,这些细胞通常具有CD73+,CD90+,CD105+,CD11b-和CD45-的标志物属性(Blood 109:4245 (2007))。人MSC表达Oct4,Sox2和Rex-1;这些mRNA均可通过RT-PCR技术进行鉴定(Arthritis Res Ther 9:204 (2007))。
什么是间充质干细胞(MSC)?
间充质干细胞(MSC)是最初从骨髓或脂肪组织中分离出来的多专能细胞,它们表现出进一步分化为骨,软骨和脂肪细胞的能力。在常规细胞培养条件下,从骨髓中分离出的MSC为纺锤形,并拥有贴附于未包被塑料培养皿上的独特能力(Arthritis Res Ther 9:204 (2007))。
How many times can I freeze/thaw the StemPro Chondrogenesis Supplement (Cat. No. A1006401) from the StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101)?
We do not recommend that you freeze/thaw the StemPro Chondrogenesis Supplement (Cat. No. A10064-01) from the StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101). Addition of supplement to the StemPro Osteocyte/Chondrocyte Differentiation Basal Medium (Cat. No. A1006901) is stable at 2-8 degrees C for up to 1 month, protected from light.
Mice lacking Nf1 in osteochondroprogenitor cells display skeletal dysplasia similar to patients with neurofibromatosis type I.
Authors:Wang W, Nyman JS, Ono K, Stevenson DA, Yang X, Elefteriou F,
Journal:Hum Mol Genet
PubMed ID:21757497
Mutations in NF1 cause neurofibromatosis type I (NF1), a disorder characterized, among other clinical manifestations, by generalized and focal bony lesions. Dystrophic scoliosis and tibial pseudoarthrosis are the most severe skeletal manifestations for which treatment is not satisfactory, emphasizing the dearth of knowledge related to the biology of NF1 in ... More
Human Synovial MSC Good Manufacturing Practices for Articular Cartilage Regeneration.
Authors:Fernandes TL, Kimura HA, Pinheiro CCG, Shimomura K, Nakamura N, Ferreira JRM, Gomoll AH, Hernandez AJ, Bueno DF,
Journal:Tissue Eng Part C Methods
PubMed ID:30412046
cartilage restoration is a desperately needed bridge for patients with symptomatic cartilage lesions. Chondral lesion is a pathology with high prevalence, reaching as much as 63% of general population and 36% among athletes. Despite Autologous Chondrocyte Implantation (ACI) versatility, it still fails to fully reproduce hyaline articular cartilage characteristics. Mesenchymal ... More