Small heat shock protein Hsp16.3 modulates its chaperone activity by adjusting the rate of oligomeric dissociation.
AuthorsFu X, Liu C, Liu Y, Feng X, Gu L, Chen X, Chang Z
JournalBiochem Biophys Res Commun
PubMed ID14521926
'Small heat shock proteins usually exist as oligomers and appear to undergo dynamic dissociation/reassociation, with oligomeric dissociation being a prerequisite for their chaperone activities. However, contradictory cases were also reported that chaperone activities could be enhanced with no change or even increase in oligomeric sizes. Using Hsp16.3 as a model ... More
Detection and quantification of biotinylated proteins using the Storm 840 Optical Scanner.
AuthorsLewis B, Rathman S, McMahon RJ
JournalJ Nutr Biochem
PubMed ID12770643
'The use of the avidin-biotin interaction is becoming an increasingly common method for the detection of proteins. The use of fluorescence detection with avidin-biotin systems has the potential to greatly increase both the sensitivity and linearity of this type of analysis. In this report, three fluorescent systems were tested for ... More
In vivo blockade of the programmed cell death-1 pathway using soluble recombinant PD-1-Fc enhances CD4+ and CD8+ T cell responses but has limited clinical benefit.
AuthorsAmancha PK, Hong JJ, Rogers K, Ansari AA, Villinger F,
Journal
PubMed ID24227774
'The programmed cell death-1 (PD-1)/programmed cell death ligand-1 pathway has been shown to limit cell-mediated effector functions during chronic viral infections impeding clearance of pathogens. As a strategy to reverse this exhaustion and increase T cell polyfunctionality, PD-1 ligands were blocked in vivo using a recombinant macaque PD-1 fused to ... More
Platelets support a protective immune response to LCMV by preventing splenic necrosis.
AuthorsLoria GD, Romagnoli PA, Moseley NB, Rucavado A, Altman JD,
JournalBlood
PubMed ID22566603
Severe arenaviral infections in humans are characterized by clinical findings common to other viral hemorrhagic fevers (VHFs), including thrombocytopenia, leukopenia, skin and internal organ hemorrhages, high viral replication, splenic necrosis, and death. Host responses, rather than direct damage by the arenaviral replication, account for most of the observed pathology, but ... More
Plasmacytoid dendritic cells are recruited to the colorectum and contribute to immune activation during pathogenic SIV infection in rhesus macaques.
AuthorsKwa S, Kannanganat S, Nigam P, Siddiqui M, Shetty RD, Armstrong W, Ansari A, Bosinger SE, Silvestri G, Amara RR,
JournalBlood
PubMed ID21693759
In SIV/HIV infection, the gastrointestinal tissue dominates as an important site because of the impact of massive mucosal CD4 depletion and immune activation-induced tissue pathology. Unlike AIDS-susceptible rhesus macaques, natural hosts do not progress to AIDS and resolve immune activation earlier. Here, we examine the role of dendritic cells (DCs) ... More
FRET and competing processes between conjugated polymer and dye substituted DNA strands: a comparative study of probe selection in DNA detection.
AuthorsAl Attar HA, Monkman AP,
JournalBiomacromolecules
PubMed ID19334782
Fluorescence resonance energy transfer (FRET) between water-soluble conjugated polymer, poly-(9,9-bis(6'-N,N,N-trimethylammonium)-hexyl-fluorene phenylene) bromide, and ssDNA's labeled with four different types of dyes (Pacific-blue, Alexa-fluor 430, Fluorescein, and ROX) has been investigated. The effect of spectral overlap and Stokes-shift on the efficiency and properties of FRET were studied. In the DNA sequence ... More
Efficiencies of fluorescence resonance energy transfer and contact-mediated quenching in oligonucleotide probes.
AuthorsMarras SA, Kramer FR, Tyagi S
JournalNucleic Acids Res
PubMed ID12409481
An important consideration in the design of oligonucleotide probes for homogeneous hybridization assays is the efficiency of energy transfer between the fluorophore and quencher used to label the probes. We have determined the efficiency of energy transfer for a large number of combinations of commonly used fluorophores and quenchers. We ... More
Structural and functional changes in the alpha A-crystallin R116C mutant in hereditary cataracts.
AuthorsCobb BA, Petrash JM
JournalBiochemistry
PubMed ID11123904
alpha-Crystallin, the major protein component of vertebrate lenses, forms a large complex comprised of two homologous subunits, alphaA- and alphaB-crystallin. It has the ability to suppress stress-induced protein aggregation in vitro, bind saturably to lens plasma membranes, and aid in light refraction through short-range ordering. Recently, a missense mutation in ... More
Acquisition of arylsulfatase A onto the mouse sperm surface during epididymal transit.
AuthorsWeerachatyanukul W, Xu H, Anupriwan A, Carmona E, Wade M, Hermo L, da Silva SM, Rippstein P, Sobhon P, Sretarugsa P, Tanphaichitr N
JournalBiol Reprod
PubMed ID12773421
Arylsulfatase A (AS-A) is localized to the sperm surface and participates in sperm-zona pellucida binding. We investigated how AS-A, usually known as an acrosomal enzyme, trafficked to the sperm surface. Immunocytochemistry of the mouse testis confirmed the existence of AS-A in the acrosomal region of round and elongating spermatids. However, ... More