Polybrene inhibits human mesenchymal stem cell proliferation during lentiviral transduction.
AuthorsLin P, Correa D, Lin Y, Caplan AI,
JournalPLoS One
PubMed ID21887340
'Human mesenchymal stem cells (hMSCs) can be engineered to express specific genes, either for their use in cell-based therapies or to track them in vivo over long periods of time. To obtain long-term expression of these genes, a lentivirus- or retrovirus-mediated cell transduction is often used. However, given that the ... More
The human GINS complex associates with Cdc45 and MCM and is essential for DNA replication.
AuthorsAparicio T, Guillou E, Coloma J, Montoya G, Méndez J,
JournalNucleic Acids Res
PubMed ID19223333
'The GINS complex, originally discovered in Saccharomyces cerevisiae and Xenopus laevis, binds to DNA replication origins shortly before the onset of S phase and travels with the replication forks after initiation. In this study we present a detailed characterization of the human GINS (hGINS) homolog. Using new antibodies that allow ... More
Rnd3 regulation of the actin cytoskeleton promotes melanoma migration and invasive outgrowth in three dimensions.
AuthorsKlein RM, Aplin AE,
JournalCancer Res
PubMed ID19244113
The depth of cell invasion into the dermis is a clinical determinant for poor prognosis in cutaneous melanoma. The signaling events that promote the switch from a noninvasive to invasive tumor phenotype remain obscure. Activating mutations in the serine/threonine kinase B-RAF are prevalent in melanoma. Mutant B-RAF is required for ... More