Alexa Fluor™ 488 C₅ Maleimide - FAQs

What are the signal intensity differences between Alexa Fluor 350 dye and Alexa Fluor 488 dye?

In general, blue fluorescent dyes are not as bright as other dyes further along the color spectrum. Blue dyes are structurally smaller and have lower extinction coefficients, so they are typically not as bright compared to the green, red, and far red dyes.
When using an Alexa Fluor 350 secondary antibody, we recommend that you use it for highly expressed targets and at a higher concentration than what is typically required for green or red secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.