Dependence of fluorescence intensity on the spectral overlap between fluorophores and plasmon resonant single silver nanoparticles.
AuthorsChen Y, Munechika K, Ginger DS
JournalNano Lett
PubMed ID17315937
'We investigate the fluorescence from dyes coupled to individual DNA-functionalized metal nanoparticles. We use single-particle darkfield scattering and fluorescence microscopy to correlate the fluorescence intensity of the dyes with the localized surface plasmon resonance (LSPR) spectra of the individual metal nanoparticles to which they are attached. For each of three ... More
Structural plasticity of an acid-activated chaperone allows promiscuous substrate binding.
AuthorsTapley TL, Körner JL, Barge MT, Hupfeld J, Schauerte JA, Gafni A, Jakob U, Bardwell JC,
JournalProc Natl Acad Sci U S A
PubMed ID19321422
'HdeA has been shown to prevent acid-induced aggregation of proteins. With a mass of only 9.7 kDa, HdeA is one of the smallest chaperones known. Unlike other molecular chaperones, which are typically complex, multimeric ATP-dependent machines, HdeA is known to undergo an acid-induced dimer to monomer transition and functions at ... More
Reversible transition between the surface trimer and membrane-inserted monomer of annexin 12.
AuthorsLadokhin AS, Haigler HT
JournalBiochemistry
PubMed ID15736950
'Under mildly acidic conditions, annexin 12 (ANX) inserts into lipid membranes to form a transbilayer pore [Langen, R., et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 14060]. In this study, we have addressed the question of the oligomeric state of ANX in this transbilayer conformation by means of Forster-type ... More
Chaperoning of insertion of membrane proteins into lipid bilayers by hemifluorinated surfactants: application to diphtheria toxin.
'Hemifluorinated compounds, such as HF-TAC, make up a novel class of nondetergent surfactants designed to keep membrane proteins soluble under nondissociating conditions [Breyton, C., et al. (2004) FEBS Lett. 564, 312]. Because fluorinated and hydrogenated chains do not mix well, supramicellar concentrations of these surfactants can coexist with intact lipid ... More
The conserved core domains of annexins A1, A2, A5, and B12 can be divided into two groups with different Ca2+-dependent membrane-binding properties.
AuthorsPatel DR, Isas JM, Ladokhin AS, Jao CC, Kim YE, Kirsch T, Langen R, Haigler HT
JournalBiochemistry
PubMed ID15723527
'The hallmark of the annexin super family of proteins is Ca(2+)-dependent binding to phospholipid bilayers, a property that resides in the conserved core domain of these proteins. Despite the structural similarity between the core domains, studies reported herein showed that annexins A1, A2, A5, and B12 could be divided into ... More
Development of a technique for the investigation of folding dynamics of single proteins for extended time periods.
AuthorsKinoshita M, Kamagata K, Maeda A, Goto Y, Komatsuzaki T, Takahashi S,
JournalProc Natl Acad Sci U S A
PubMed ID17563378
A technique was developed for the detection of fluorescence signals from free single molecules for extended time periods and was applied to the characterization of the unfolded states of iso-1-cytochrome c (cyt c). Protein molecules labeled with fluorescent dye were slowly injected into a capillary at concentrations that allow for ... More
Caspase-3 sensitive signaling in vivo in apoptotic HeLa cells by chemically engineered intramolecular fluorescence resonance energy transfer mutants of green fluorescent protein.
AuthorsSuzuki M, Ito Y, Sakata I, Sakai T, Husimi Y, Douglas KT,
JournalBiochem Biophys Res Commun
PubMed ID15796904
Green fluorescent protein (UV5) was re-engineered to remove native cysteine residues, and a new cysteine was introduced near the C-terminus, approximately 20 A from the native fluorophore, for site-specific attachment of chemical fluorophores. The resultant efficient intramolecular FRET quenched GFP emission and gave a new emission band from the conjugated ... More