A multifunctional bioconjugate module for versatile photoaffinity labeling and click chemistry of RNA.
Authors:Kellner S, Seidu-Larry S, Burhenne J, Motorin Y, Helm M,
Journal:Nucleic Acids Res
PubMed ID:21646334
A multifunctional reagent based on a coumarin scaffold was developed for derivatization of naive RNA. The alkylating agent N3BC [7-azido-4-(bromomethyl)coumarin], obtained by Pechmann condensation, is selective for uridine. N3BC and its RNA conjugates are pre-fluorophores which permits controlled modular and stepwise RNA derivatization. The success of RNA alkylation by N3BC ... More