SuperScript® hESC Full-Length Normalized cDNA Library, BG03 - FAQs

What is the best way to prepare a plasmid prep of an Invitrogen premade library in order to get the highest representation of clones for transfection of mammalian cells?

The key to minimizing a change in library representation is to put the library through as few cell divisions as possible. Initiate a culture with as much of the frozen library as possible. Growing up the library from a very small number of starting cells is not ideal since a large number of cell divisions will be required to reach the appropriate density. Do not amplify the library because this will also change the representation.