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View additional product information for ArC™ Amine Reactive Compensation Bead Kit (for use with LIVE/DEAD™ Fixable dead cell stain kits) - FAQs (A10346, A10628)
17 product FAQs found
这是可能的。但是如果您的值超过100，可能需要查看你们用来采集数据的电压。
通过运行单色对照，有可能除去溢出到另一个荧光采集通道的荧光信号。
进行补偿时，你需要为自己使用的每种颜色参数准备单一染色样品（或补偿微球）。此外，我们建议您使用FMO（荧光扣除）对照。您可以使用减去一种颜色的其他所有颜色标记您的细胞或者微球来作为对照。为各种颜色准备FMO对照。这些对照对正确设置数据门限很有帮助。
需要，您需要为您试验中任何一种颜色设置补偿对照。诸如我们LIVE/DEAD Fixable Dead Cell Stain Kits这样的胺基反应染色，您需要使用我们的ArC Amine Reactive Compensation Bead Kit（货号 A10346）标记珠子设置补偿对照。对于其他染色，例如细胞周期染色，您需要为您的补偿对照使用标记细胞。
理想情况下，每一个单独荧光团的荧光发射光谱都非常集中，窄峰，发射峰之间分隔的较开。现实中，有机染料和荧光蛋白有很宽的发射峰，必须考虑补偿（数据采集中或后）来矫正任何一个荧光团荧光信号的分配。补偿从重叠光谱中移除了荧光信号，使您知道所看见的信号就是目标荧光团发出的信号，所以非常重要。
补偿对照仅需未染色的细胞样品（或阴性对照）和单一颜色标记的样品——每管一个。如果您希望单色染色对照如您期望的那么亮,，那么可以使用我们的AbC总抗体补偿微珠试剂盒（货号A10513和A10497），这试剂盒是将抗体结合到微珠而非细胞上。
While the polypropylene cap is compatible with Vaporized Hydrogen Peroxide (VHP) sterilization, we have not tested the compatibility of the Low-Density Polyethylene (LDPE) / Linear Low-Density Polyethylene (LLDPE) vials with VHP sterilization.
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When stored as directed (2-8 degrees C), this kit is stable for at least 1 year. Please do not freeze the kit. Please visit the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/arc_amine_reactive_comp_bead_kit_man.pdf) for further technical details.
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This can occur if the solution of beads was ever frozen. The formation of ice excludes the beads and compresses the beads together. Once frozen, the beads cannot be resuspended into solution and are no longer usable.
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This is because succinimidyl ester (NHS ester) groups are very labile to water; they dissociate readily from the dye structure if held in solvents that are contaminated with water, as is possible from condensation or absorbing water from the atmosphere.
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Yes. The succinimidyl ester (NHS ester) and isothiocyanate (e.g., FITC, TRITC) dye derivatives may be attached to the ArC beads, but you would have to optimize the amount of reactive dye per ArC beads.
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It is possible. But if you have a lot of values over 100, you probably need to look at the voltages that you are using for your data collection.
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By running single-color controls, it is possible to remove signal of one fluorophore that spills over into the collection channel for another fluorophore.
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For compensation, you need to prepare a singly stained sample (or compensation beads) for each color parameter that you are using. In addition, we recommend that you use FMO (flow minus one) controls. These are controls in which you label cells or beads with every color in your panel, omitting one. Make one FMO control for each color. These controls are important for helping you properly set gates on your data.
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Yes, you should have a compensation control for each color in your assay. For amine-reactive stains such as our LIVE/DEAD Fixable Dead Cell Stain Kits, you can label beads to make compensation controls using our ArC Amine Reactive Compensation Bead Kit (Cat. No. A10346). For other stains, such as cell cycle stains, you need to use labeled cells for your compensation control.
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In a perfect world, the fluorescence emission profile for each individual fluorophore would be a very intense, narrow peak, well separated from all other emission peaks. In reality, organic dyes and fluorescent proteins have broad emission peaks, and compensation must be employed (during or after data acquisition) to correctly assign fluorescence signal to each fluorophore. Compensation is important because it removes fluorescent signal from overlapping spectra so you know that the signal you see is only the signal from the fluorophore of interest.
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All that you need for compensation controls is an unstained sample of your cells (or negative control beads) and samples with each of your fluorophores - one per tube. You want the single-stained controls to be as bright as you expect your brightest sample to be. Antibodies can be bound to beads instead of cells using our AbC Total Antibody Compensation Bead Kit (Cat. Nos. A10513 and A10497).
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