Inhibitory mechanisms that generate centre and surround properties in ON and OFF brisk-sustained ganglion cells in the rabbit retina.
AuthorsBuldyrev I, Taylor WR,
JournalJ Physiol
PubMed ID23045347
Abstract:Lateral inhibition produces the centre-surround organization of retinal receptive fields, in which inhibition driven by the mean luminance enhances the ... More
Site-specific modification of AAV vector particles with biophysical probes and targeting ligands using biotin ligase.
AuthorsStachler MD, Chen I, Ting AY, Bartlett JS,
JournalMol Ther
PubMed ID18560418
Abstract:'We have developed a highly specific and robust new method for labeling adeno-associated virus (AAV) vector particles with either biophysical ... More
Altered connexin expression and wound healing in the epidermis of connexin-deficient mice.
AuthorsKretz M, Euwens C, Hombach S, Eckardt D, Teubner B, Traub O, Willecke K, Ott T
JournalJ Cell Sci
PubMed ID12840073
Abstract:'To analyze the effect of connexin loss on the repair of wounded tail skin, we have studied the following transgenic ... More
Gap junctional communication in the early Xenopus embryo.
AuthorsLandesman Y, Goodenough DA, Paul DL
JournalJ Cell Biol
PubMed ID10953017
Abstract:'In the Xenopus embryo, blastomeres are joined by gap junctions that allow the movement of small molecules between neighboring cells. ... More
Characterization of a synaptiform transmission between a neuron and a glial cell in the leech central nervous system.
AuthorsBritz FC, Lohr C, Schmidt J, Deitmer JW
JournalGlia
PubMed ID11968059
Abstract:'The cross-talk between neurons and glial cells is receiving increased attention because of its potential role in information processing in ... More
Intracellular fluid flow in rapidly moving cells.
AuthorsKeren K, Yam PT, Kinkhabwala A, Mogilner A, Theriot JA,
JournalNat Cell Biol
PubMed ID19767741
Abstract:'Cytosolic fluid dynamics have been implicated in cell motility because of the hydrodynamic forces they induce and because of their ... More
Quantitative analysis of gap-junctional intercellular communication in precision-cut mouse liver slices.
AuthorsRomualdi A, Niessen H, Dombrowski F, Willecke K, Ott T
JournalCell Tissue Res
PubMed ID11904767
Abstract:'Direct intercellular communication through gap junction channels is involved in the maintenance of tissue homeostasis and suppression of carcinogenesis. Gap-junctional ... More
Long-term stimulation of mouse hippocampal slice culture on microelectrode array.
Authorsvan Bergen A, Papanikolaou T, Schuker A, Möller A, Schlosshauer B
JournalBrain Res Brain Res Protoc
PubMed ID12738008
Abstract:'To understand mechanisms of information processing, development and degeneration of the central nervous system, simultaneous multisite recording and stimulation have ... More
A novel role for MNTB neuron dendrites in regulating action potential amplitude and cell excitability during repetitive firing.
AuthorsLeão RN, Leão RM, da Costa LF, Rock Levinson S, Walmsley B,
JournalEur J Neurosci
PubMed ID18598256
Abstract:'Principal cells of the medial nucleus of the trapezoid body (MNTB) are simple round neurons that receive a large excitatory ... More
Quantification of carbonylated proteins in rat skeletal muscle mitochondria using capillary sieving electrophoresis with laser-induced fluorescence detection.
AuthorsFeng J, Arriaga EA,
JournalElectrophoresis
PubMed ID18064596
Abstract:'Carbonyl-modified proteins are markers of oxidative damage. Here, we report a new method for detecting and quantifying carbonylated proteins by ... More
Functional localization of neurotransmitter receptors and synaptic inputs to mature neurons of the medial superior olive.
AuthorsCouchman K, Grothe B, Felmy F,
JournalJ Neurophysiol
PubMed ID22131383
Abstract:'Neurons of the medial superior olive (MSO) code for the azimuthal location of low-frequency sound sources via a binaural coincidence ... More
T-type Ca(2+) channels mediate neurotransmitter release in retinal bipolar cells.
AuthorsPan ZH, Hu HJ, Perring P, Andrade R
JournalNeuron
PubMed ID11604141
Abstract:'Transmitter release in neurons is thought to be mediated exclusively by high-voltage-activated (HVA) Ca(2+) channels. However, we now report that, ... More
Developmental profile and properties of sulforhodamine 101--Labeled glial cells in acute brain slices of rat hippocampus.
AuthorsKafitz KW, Meier SD, Stephan J, Rose CR,
JournalJ Neurosci Methods
PubMed ID18187203
Abstract:'The reliable identification of astrocytes for physiological measurements was always time-consuming and difficult. Recently, the fluorescent dye sulforhodamine 101 (SR101) ... More
Oxidation-triggered release of fluorescent molecules or drugs from mesoporous Si microparticles.
AuthorsWu EC, Park JH, Park J, Segal E, Cunin F, Sailor MJ,
JournalACS Nano
PubMed ID19206408
Abstract:'The fluorescent dye Alexa Fluor 488 or the anticancer drug doxorubicin is attached to the surface and inner pore walls ... More
Luteinizing hormone causes MAP kinase-dependent phosphorylation and closure of connexin 43 gap junctions in mouse ovarian follicles: one of two paths to meiotic resumption.
AuthorsNorris RP, Freudzon M, Mehlmann LM, Cowan AE, Simon AM, Paul DL, Lampe PD, Jaffe LA,
JournalDevelopment
PubMed ID18776144
Abstract:'Luteinizing hormone (LH) acts on ovarian follicles to reinitiate meiosis in prophase-arrested mammalian oocytes, and this has been proposed to ... More
Saposin C is required for lipid presentation by human CD1b.
AuthorsWinau F, Schwierzeck V, Hurwitz R, Remmel N, Sieling PA, Modlin RL, Porcelli SA, Brinkmann V, Sugita M, Sandhoff K, Kaufmann SH, Schaible UE
JournalNat Immunol
PubMed ID14716313
Abstract:'Lipids from Mycobacterium tuberculosis are presented through CD1 proteins to T lymphocytes in humans, but the accessory molecules required for ... More
Protein-heparin interactions measured by BIAcore 2000 are affected by the method of heparin immobilization.
AuthorsOsmond RI, Kett WC, Skett SE, Coombe DR
JournalAnal Biochem
PubMed ID12423639
Abstract:'Surface plasmon resonance (SPR) biosensors such as the BIAcore 2000 are a useful tool for the analysis of protein-heparin interactions. ... More
Bipolar cells use kainate and AMPA receptors to filter visual information into separate channels.
AuthorsDeVries SH
JournalNeuron
PubMed ID11163271
Abstract:Unlike cone photoreceptors, whose light responses have a uniform time course, retinal ganglion cells are tuned to respond to different ... More
Transformation of olfactory representations in the Drosophila antennal lobe.
AuthorsWilson RI, Turner GC, Laurent G
JournalScience
PubMed ID14684826
Abstract:Molecular genetics has revealed a precise stereotypy in the projection of primary olfactory sensory neurons onto secondary neurons. A major ... More
Monitoring presynaptic calcium dynamics in projection fibers by in vivo loading of a novel calcium indicator.
AuthorsKreitzer AC, Gee KR, Archer EA, Regehr WG
JournalNeuron
PubMed ID10939328
Abstract:Fluorometric calcium measurements have revealed presynaptic residual calcium (Ca(res)) to be an important regulator of synaptic strength. However, in the ... More
Mechanisms of quenching of Alexa fluorophores by natural amino acids.
Abstract:Quenching of fluorophores by the same proteins that they covalently label is a phenomenon that is neither well-known nor well-characterized. ... More
Hindered diffusion through an aqueous pore describes invariant dye selectivity of Cx43 junctions.
AuthorsHeyman NS, Burt JM,
JournalBiophys J
PubMed ID17921206
Abstract:The permselectivity (permeance/conductance) of Cx43-comprised gap junctions is a variable parameter of junctional function. To ascertain whether this variability in ... More
Form and function of the M4 cell, an intrinsically photosensitive retinal ganglion cell type contributing to geniculocortical vision.
AuthorsEstevez ME, Fogerson PM, Ilardi MC, Borghuis BG, Chan E, Weng S, Auferkorte ON, Demb JB, Berson DM,
JournalJ Neurosci
PubMed ID23015450
Abstract:The photopigment melanopsin confers photosensitivity upon a minority of retinal output neurons. These intrinsically photosensitive retinal ganglion cells (ipRGCs) are ... More
Effects of axonal topology on the somatic modulation of synaptic outputs.
AuthorsSasaki T, Matsuki N, Ikegaya Y,
JournalJ Neurosci
PubMed ID22357869
Abstract:Depolarization of the neuronal soma augments synaptic output onto postsynaptic neurons via long-range, axonal cable properties. Here, we report that ... More
eGFP Expression under UCHL1 Promoter Genetically Labels Corticospinal Motor Neurons and a Subpopulation of Degeneration-Resistant Spinal Motor Neurons in an ALS Mouse Model.
AuthorsYasvoina MV, Genç B, Jara JH, Sheets PL, Quinlan KA, Milosevic A, Shepherd GM, Heckman CJ, Ozdinler PH,
JournalJ Neurosci
PubMed ID23637180
Abstract:Understanding mechanisms that lead to selective motor neuron degeneration requires visualization and cellular identification of vulnerable neurons. Here we report ... More
Tonic NMDA receptor-mediated current in prefrontal cortical pyramidal cells and fast-spiking interneurons.
AuthorsPovysheva NV, Johnson JW,
JournalJ Neurophysiol
PubMed ID22236713
Abstract:Tonically activated neuronal currents mediated by N-methyl-d-aspartate receptors (NMDARs) have been hypothesized to contribute to normal neuronal function as well ... More
Compartmentalized dendritic plasticity and input feature storage in neurons.
AuthorsLosonczy A, Makara JK, Magee JC,
JournalNature
PubMed ID18368112
Abstract:Although information storage in the central nervous system is thought to be primarily mediated by various forms of synaptic plasticity, ... More
Hierarchical connectivity and connection-specific dynamics in the corticospinal-corticostriatal microcircuit in mouse motor cortex.
AuthorsKiritani T, Wickersham IR, Seung HS, Shepherd GM,
JournalJ Neurosci
PubMed ID22492054
Abstract:The generation of purposive movement by mammals involves coordinated activity in the corticospinal and corticostriatal systems, which are involved in ... More
Dye-permeable, voltage-gated channel on mouse fungiform taste bud cells.
AuthorsTakeuchi K, Seto Y, Ohtubo Y, Yoshii K,
JournalBrain Res
PubMed ID21167135
Abstract:We show here the expression, permeability and pharmacology of a voltage-gated channel in certain taste bud cells (TBCs) which is ... More
Parameters underlying distinct T cell-dependent polysaccharide-specific IgG responses to an intact gram-positive bacterium versus a soluble conjugate vaccine.
AuthorsColino J, Chattopadhyay G, Sen G, Chen Q, Lees A, Canaday DH, Rubtsov A, Torres R, Snapper CM,
JournalJ Immunol
PubMed ID19570830
Abstract:IgG anti-polysaccharide (PS) responses to both intact Streptococcus pneumoniae (Pn) and PS conjugate vaccines are dependent on CD4(+) T cells, ... More
Determination of absolute protein numbers in single synapses by a GFP-based calibration technique.
AuthorsSugiyama Y, Kawabata I, Sobue K, Okabe S,
JournalNat Methods
PubMed ID16118638
Abstract:To build a quantitative model of molecular organization of neurons, it is essential to have information about the number of ... More
Advantages and limitations of commonly used methods to assay the molecular permeability of gap junctional intercellular communication.
AuthorsAbbaci M, Barberi-Heyob M, Blondel W, Guillemin F, Didelon J,
JournalBiotechniques
PubMed ID18611167
Abstract:The role of gap junctional intercellular communication (GJIC) in regulation of normal growth and differentiation is becoming increasingly recognized as ... More
Light-directed electrical stimulation of neurons cultured on silicon wafers.
AuthorsStarovoytov A, Choi J, Seung HS
JournalJ Neurophysiol
PubMed ID15385589
Abstract:Dissociated neurons cultured in vitro can serve as a model system for studying the dynamics of neural networks. Such studies ... More
The spine neck filters membrane potentials.
AuthorsAraya R, Jiang J, Eisenthal KB, Yuste R
JournalProc Natl Acad Sci U S A
PubMed ID17093040
Abstract:Dendritic spines receive most synaptic inputs in the forebrain. Their morphology, with a spine head isolated from the dendrite by ... More
Lipopolysaccharide (LPS) labeled with Alexa 488 hydrazide as a novel probe for LPS binding studies.
AuthorsTriantafilou K, Triantafilou M, Fernandez N
JournalCytometry
PubMed ID11084617
Abstract:BACKGROUND: Lipopolysaccharide (LPS) comprises the outer cell wall of all gram-negative bacteria. It consists of an oligosaccharide core and lipid ... More
Two phases of zymogen granule lifetime in mouse pancreas: ghost granules linger after exocytosis of contents.
AuthorsThorn P, Parker I
JournalJ Physiol
PubMed ID15637100
Abstract:Different cell types show widely divergent mechanisms and kinetics of exocytosis. We investigated these processes in pancreatic acinar cells by ... More
Detecting and quantifying colocalization of cell surface molecules by single particle fluorescence imaging.
AuthorsMorrison IE, Karakikes I, Barber RE, Fernández N, Cherry RJ
JournalBiophys J
PubMed ID14645098
Abstract:Single particle fluorescence imaging (SPFI) uses the high sensitivity of fluorescence to visualize individual molecules that have been selectively labeled ... More
Folding of A+U-rich RNA elements modulates AUF1 binding. Potential roles in regulation of mRNA turnover.
AuthorsWilson GM, Sutphen K, Chuang Ky Brewer G
JournalJ Biol Chem
PubMed ID11124962
Abstract:In mammals, A+U-rich elements (AREs) are potent cis-acting determinants of rapid cytoplasmic mRNA turnover. Recognition of these sequences by AUF1 ... More
Fluid-phase endocytosis does not contribute to rapid fluid secretion in the malpighian tubules of the house cricket, Acheta domesticus.
AuthorsHazelton SR, Spring JH, Felgenhauer BE
JournalJ Exp Zool
PubMed ID11754017
Abstract:When the Malpighian tubules (Mt) of the house cricket (Acheta domesticus) are treated with dibutyryl adenosine 3', 5'-cyclic monophosphate (db-cAMP; ... More
Quantitative comparisons of corticothalamic topography within the ventrobasal complex and the posterior nucleus of the rodent thalamus.
AuthorsAlloway KD, Hoffer ZS, Hoover JE
JournalBrain Res
PubMed ID12644264
Abstract:To compare the topographic precision of corticothalamic projections to the ventrobasal (VB) complex and the medial part of the posterior ... More
The permeability of gap junction channels to probes of different size is dependent on connexin composition and permeant-pore affinities.
Abstract:Gap junctions have traditionally been characterized as nonspecific pores between cells passing molecules up to 1 kDa in molecular mass. ... More
A transient diffusion model yields unitary gap junctional permeabilities from images of cell-to-cell fluorescent dye transfer between Xenopus oocytes.
Abstract:As ubiquitous conduits for intercellular transport and communication, gap junctional pores have been the subject of numerous investigations aimed at ... More
Dendritic compartmentalization of chloride cotransporters underlies directional responses of starburst amacrine cells in retina.
AuthorsGavrikov KE, Nilson JE, Dmitriev AV, Zucker CL, Mangel SC
JournalProc Natl Acad Sci U S A
PubMed ID17124178
Abstract:The mechanisms in the retina that generate light responses selective for the direction of image motion remain unresolved. Recent evidence ... More
Conductance and permeability of the residual state of connexin43 gap junction channels.
AuthorsBukauskas FF, Bukauskiene A, Verselis VK
JournalJ Gen Physiol
PubMed ID11815667
Abstract:We used cell lines expressing wild-type connexin43 and connexin43 fused with the enhanced green fluorescent protein (Cx43-EGFP) to examine conductance ... More
Quality control procedures for dose-response curve generation using nanoliter dispense technologies.
AuthorsQuintero C, Rosenstein C, Hughes B, Middleton R, Kariv I
JournalJ Biomol Screen
PubMed ID17517899
Abstract:With the advancement of high-throughput biomolecular screening techniques to the lead optimization stage, there is a critical need to quality ... More
Permeability and gating properties of human connexins 26 and 30 expressed in HeLa cells.
AuthorsBeltramello M, Bicego M, Piazza V, Ciubotaru CD, Mammano F, D'Andrea P
JournalBiochem Biophys Res Commun
PubMed ID12767933
Abstract:Human connexins 26 and 30 were expressed either through the bicistronic pIRES-EGFP expression vector or as EYFP-tagged chimeras. When transiently ... More
Mutation of a conserved threonine in the third transmembrane helix of alpha- and beta-connexins creates a dominant-negative closed gap junction channel.
AuthorsBeahm DL, Oshima A, Gaietta GM, Hand GM, Smock AE, Zucker SN, Toloue MM, Chandrasekhar A, Nicholson BJ, Sosinsky GE
JournalJ Biol Chem
PubMed ID16407179
Abstract:Single site mutations in connexins have provided insights about the influence specific amino acids have on gap junction synthesis, assembly, ... More
Endocannabinoid-independent retrograde signaling at inhibitory synapses in layer 2/3 of neocortex: involvement of vesicular glutamate transporter 3.
Abstract:Recent studies implicate dendritic endocannabinoid release from subsynaptic dendrites and subsequent inhibition of neurotransmitter release from nerve terminals as a ... More
Intracellular pathways regulating ciliary beating of rat brain ependymal cells.
AuthorsNguyen T, Chin WC, O'Brien JA, Verdugo P, Berger AJ
JournalJ Physiol
PubMed ID11179397
Abstract:1. The mammalian brain ventricles are lined with ciliated ependymal cells. As yet little is known about the mechanisms by ... More
Calcium influx into dendrites of the leech Retzius neuron evoked by 5-hydroxytryptamine.
AuthorsBeck A, Lohr C, Berthold H, Deitmer JW
JournalCell Calcium
PubMed ID12027387
Abstract:5-Hydroxytryptamine (5-HT) is a ubiquitous neurotransmitter and neuromodulator that affects neural circuits and behaviours in vertebrates and invertebrates. In the ... More