Alexa Fluor™ 594 酰肼，用于显微注射
Alexa Fluor™ 594 酰肼，用于显微注射
引用和文献 (61)
Invitrogen™

Alexa Fluor™ 594 酰肼，用于显微注射

Alexa Fluor™ 594 酰肼是一种细胞示踪剂，也是一种用于标记多糖或糖蛋白中醛或酮的反应性染料。该版本以即用型溶液的形式提供，溶解在 200 mM KCl 溶液中并经过除菌过滤。Alexa了解更多信息
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货号数量
A10442
又称 A-10442
125 μL
货号 A10442
又称 A-10442
价格（CNY)
5,421.00
Each
添加至购物车
数量:
125 μL
价格（CNY)
5,421.00
Each
添加至购物车
Alexa Fluor™ 594 酰肼是一种细胞示踪剂，也是一种用于标记多糖或糖蛋白中醛或酮的反应性染料。该版本以即用型溶液的形式提供，溶解在 200 mM KCl 溶液中并经过除菌过滤。

Alexa Fluor™ 594 是一种明亮的红色荧光染料。Alexa Fluor™ 594 染料用于成像和流式细胞分析中稳定信号的生成，具有水溶性和 pH 值不敏感性（pH 值 4 至 pH 值 10）。除反应性染料制剂外，我们还提供可与多种抗体、肽、蛋白、示踪剂和扩增底物偶联并且针对细胞标记和检测进行优化的 Alexa Fluor™ 594 染料（了解更多信息）。

关于该 AlexaFluor™ 酰肼的详细信息：

•荧光基团标记：Alexa Fluor™ 594 染料
•反应性基团：酰肼
•反应性：多糖或糖蛋白中的醛或酮
•偶联物的 Ex/Em：588/613 nm
• 消光系数：97,000 cm-1M-1
• 光谱相似染料：Texas Red
• 分子量：758.79

细胞示踪和示踪应用
Alexa Fluor™ 酰肼和羟胺是低分子量、膜不可透过、可用醛固定的细胞示踪剂，与由其他在光谱上类似的荧光基团形成的细胞示踪剂相比，具有更明亮的荧光和更强的光稳定性。它们可通过显微注射、从膜片移液管注入或通过我们的 Influx™ 胞饮细胞上样试剂所诱导的摄取进入细胞。了解更多有关细胞追踪和示踪的信息。

糖蛋白和多糖标记应用
Alexa Fluor™ 酰肼和羟胺是反应性分子，可用于向含有醛或酮的生物分子添加荧光标记。醛和酮可通过高碘酸盐介导的邻二醇氧化而引入多糖和糖蛋白中。半乳糖氧化酶也可用于将糖蛋白的末端半乳糖残基氧化为醛。

酰肼与羟胺
肼衍生物与酮和醛反应从而生成相对稳定的腙。羟胺衍生物（氨基氧基化合物）与醛和酮反应生成肟。肟的水解稳定性优于腙。使用硼氢化钠 (NaBH4) 可以还原腙和肟，以进一步提高键的稳定性。

了解有关蛋白和抗体标记的更多信息
我们可提供多种可供选择的 Molecular Probes ™ 抗体和蛋白标记试剂盒，以适应您的起始料和实验设置。参见我们的抗体标记试剂盒或使用我们的标记化学选择工具进行其他选择。欲了解有关我们标记试剂盒的更多信息，请参阅 Molecular Probes™ 手册中第 1.2 节—蛋白和核酸标记试剂盒。

我们还’可为您定制偶联物
如果您’无法在我们的在线目录中找到’想要的产品，我们还’可为您定制抗体或蛋白偶联物。我们的定制偶联服务是高效和保密的，我们保证我们的工作质量。我们经过 ISO 9001:2000 认证。

相关产品
DMSO（二甲亚砜）(D12345)
0.5-1 mg 用抗体偶联物纯化试剂盒 (A33086)
20-50 µg 用抗体偶联物纯化试剂盒 (A33087)
50-100 µg 用抗体偶联物纯化试剂盒 (A33088)
仅供科研使用。不可用于诊断程序。
规格
化学反应性羧酸、酮、醛
发射613 nm
激发588 nm
标签或染料Alexa Fluor™ 594
产品类型酰肼
数量125 μL
反应一部分胺、酰肼
运输条件室温
标签类型Alexa Fluor 染料
产品线Alexa Fluor
Unit SizeEach
内容与储存
储存在冰箱(-5 至 -30°C)中并避光。

常见问题解答 (FAQ)

What dye can I use that is non-reactive and can show an injection site?

The non-reactive Alexa Fluor and Alexa Fluor hydrazide derivatives may be used for injection site visualization. Other options include the fluorescent polystyrene microspheres, FluoSpheres, and dye-conjugated dextrans. The hydrazide derivatives and 'fixable' dextrans are retained by cross-linking using an aldehyde-based fixative.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (61)

引用和文献
Abstract
Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging.
Authors:Yasuda R, Harvey CD, Zhong H, Sobczyk A, van Aelst L, Svoboda K
Journal:Nat Neurosci
PubMed ID:16429133
'To understand the biochemical signals regulated by neural activity, it is necessary to measure protein-protein interactions and enzymatic activity in neuronal microcompartments such as axons, dendrites and their spines. We combined two-photon excitation laser scanning with fluorescence lifetime imaging to measure fluorescence resonance energy transfer at high resolutions in brain ... More
Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates.
Authors:Panchuk-Voloshina N, Haugland RP, Bishop-Stewart J, Bhalgat MK, Millard PJ, Mao F, Leung WY, Haugland RP
Journal:J Histochem Cytochem
PubMed ID:10449539
'Alexa 350, Alexa 430, Alexa 488, Alexa 532, Alexa 546, Alexa 568, and Alexa 594 dyes are a new series of fluorescent dyes with emission/excitation spectra similar to those of AMCA, Lucifer Yellow, fluorescein, rhodamine 6G, tetramethylrhodamine or Cy3, lissamine rhodamine B, and Texas Red, respectively (the numbers in the ... More
Rapid and reversible chemical inactivation of synaptic transmission in genetically targeted neurons.
Authors:Karpova AY, Tervo DG, Gray NW, Svoboda K
Journal:Neuron
PubMed ID:16337911
'Inducible and reversible silencing of selected neurons in vivo is critical to understanding the structure and dynamics of brain circuits. We have developed Molecules for Inactivation of Synaptic Transmission (MISTs) that can be genetically targeted to allow the reversible inactivation of neurotransmitter release. MISTs consist of modified presynaptic proteins that ... More
The kinetics of phagosome maturation as a function of phagosome/lysosome fusion and acquisition of hydrolytic activity.
Authors:Yates RM, Hermetter A, Russell DG
Journal:Traffic
PubMed ID:15813751
'Professional phagocytes function at the hinge of innate and acquired immune responses by internalizing particulate material that is digested and sampled within the phagosome of the cell. Despite intense interest, assays to measure phagosome maturation remain insensitive and few in number. In this current study, we describe three novel assays ... More
Intracellular fluid flow in rapidly moving cells.
Authors:Keren K, Yam PT, Kinkhabwala A, Mogilner A, Theriot JA,
Journal:Nat Cell Biol
PubMed ID:19767741
'Cytosolic fluid dynamics have been implicated in cell motility because of the hydrodynamic forces they induce and because of their influence on transport of components of the actin machinery to the leading edge. To investigate the existence and the direction of fluid flow in rapidly moving cells, we introduced inert ... More
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