CTS™ OpTmizer™ T Cell Expansion SFM, bottle format - FAQs

View additional product information for CTS™ OpTmizer™ T Cell Expansion SFM, bottle format - FAQs (A1048501)

8 product FAQs found

What is CTS?

The Gibco Cell Therapy Systems (CTS) portfolio of cell and gene therapy products are GMP manufactured, safety tested, and backed by regulatory documentation to support your transition from discovery through clinical and commercial manufacturing. Through our CTS solutions, we are committed to helping customers streamline therapeutic development, minimize risk, and ease the burden on their quality systems. Learn more here.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I need to order additional components with the CTS OpTmizer T Cell Expansion SFM (Cat. Nos. A1048501, A1048502, A1048503)?

The CTS OpTmizer T Cell Expansion SFM (Cat. Nos. A1048501, A1048502, A1048503) kit comes with OpTmizer T Cell Expansion Basal Medium and OpTmizer T Cell Expansion Supplement, which are mixed together prior to use. Depending on your protocol you may need to purchase T cell growth factors such as IL-2 (Cat. No. PHC0023) and L-glutamine (Cat. No. 25030) to form a complete medium. To boost growth of human T cells, human AB serum can be added. If desired, antibiotics such as Gentamicin (Cat. No. 15750), can also be added.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will CTS OpTmizer T Cell Expansion SFM (Cat. No. A1048501, A1048502, A1048503) work with all types of T cells?

CTS OpTmizer T Cell Expansion SFM (Cat. No. A1048501, A1048502, A1048503) has been demonstrated to be effective for the ex vivo expansion of human CD3 T cells, including polyclonal T cell populations, antigen-specific T cells, tumor-infiltrating lymphocytes (TIL), cord blood T cells, regulatory T cells, T cells from HIV-infected donors, gene-modified T cells (both lenti- and MLV-based vectors), including cells of both the CD4+ and CD8+ phenotype.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can CTS OpTmizer T Cell Expansion SFM (Cat. Nos. A1048501, A1048502, A1048503) be used for animal T cell cultures other than human?

CTS OpTmizer T Cell Expansion SFM (Cat. No. A1048501, A1048502, A1048503) has only been tested on human T cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is there a difference in formulation of the CTS OpTmizer T Cell Expansion SFM between the bottle and bag packaging formats?

The bottle and bag packaging formats of the medium have the same formulation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use CTS OpTmizer T Cell Expansion SFM for culture and expansion of mouse T cells? If not, which medium would you recommend?

We have not tested this medium on mouse T cells. If you're looking for a serum-free medium to culture mouse T cells, you can try AIM V Medium (Cat. No. 12055083 or 12055091). For mouse cells, we recommend adding 50 µM beta-mercaptoethanol (Cat. No. 21985023).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can GlutaMAX be used instead of Glutamine to supplement CTS OpTmizer T-Cell Expansion SFM?

GlutaMAX cannot replace Glutamine because cells may not initially use GlutaMAX so that T cells cannot grow. We recommend adding 4 mM GlutaMAX and 2 mM Glutamine (as stated in the manual) into the CTS OpTmizer T-Cell Expansion SFM if GlutaMAX is needed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of CTS OpTmizer T-Cell Expansion SFM?

The complete CTS OpTmizer T-Cell Expansion SFM is stable for 4 weeks when stored in the dark at 2-8 degrees C. However, the two individual medium components are stable for 18 months when stored in the dark at 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.