• 200 μL SequalPrep™ Long 聚合酶 (5 U/μL) • 1.2 ml SequalPrep™ 10X 反应缓冲液 • 1.2 mL SequalPrep™ 10X 增强剂 A • 1.2 mL SequalPrep™ 10X 增强剂 B • 750 μL DMSO
在 -20°C 下储存。DMSO 可在 4°C 或室温下储存。
常见问题解答 (FAQ)
What is the fidelity of the SequalPrep Long Polymerase in SequalPrep Long PCR Kit with dNTPs?
The SequalPrep Long PCR Kit with dNTPs demonstrates fidelity 3-4 times greater than recombinant Taq polymerase, similar to Invitrogen’s Platinum Taq High Fidelity Enzyme (by rpsl assay). The SequalPrep Long PCR Polymerase was compared to rTaq, Platinum Taq HiFi, & Roche's Expand Long Template PCR System. Relative fidelity:
• rTaq = 1
• Platinum Taq HiFi = 3.3
• Roche = 3.6
• SequalPrep Long with Enhancer A = 3.6
• SequalPrep Long with Enhancer B = 4.4
SequalPrep Long PCR Kit with dNTPs comes with several enhancers. What are they and when should I use them?
The two Enhancers were selected to help provide maximum success rates. We recommend setting up the initial reactions with 0.5X Enhancer A to provide maximum success rate. For any unsuccessful amplicons we recommend testing the following conditions to optimize amplification:
• 1.0X Enhancer A
• 0.5X Enhancer B
• 1.0X Enhancer B
What amount of input material do you recommend for SequalPrep Long PCR Kit with dNTPs?
We recommend 5-200 ng of human genomic DNA and 20-80 ng of mouse embryonic stem cells DNA. 50 ng of DNA template at a concentration of 50 ng/µl should be sufficient for most applications, although input amounts as low as 10 ng can be possible depending on the sample type, due to the high yields generated by the kit.