RiboMinus™ Eukaryote Kit for RNA-Seq - FAQs

对于物种特异性来说，以下物种对于LSU/SSU探针没有任何错配(LSU (28S) / SSU (18S)探针完美配对）。这意味着探针可从下列物种中去除18S和28S序列。

陆生物种：Human, mouse, rat, frog, rabbit, cow, pig, chicken, Drosophila,* Caenorhabditis elegans, midge, mosquito, yeast (Saccharomyces cerevisiae, Schizosaccharomyces pombe)
海洋物种：Sea squirt, coelacanth, lancelet, eel, salmon, sturgeon, ratfish, lamprey, brown plankton, zooplankton
植物：Arabidopsis thaliana, Brassica napus, rice, tomato, Humulus lupulus, Zea mays, wheat, soybean, pine, aspen

* 该试剂盒中的28S和18S探针分别和果蝇的 18S和28S rRNA序列完全匹配。但是，有报道表明果蝇(昆虫)的 28S rRNA会分裂为2个较小片段并与18S共迁移。目前准确的分裂位点还未被找到。但是，基于用户的反馈，该试剂盒并不能完全去除分裂的28S rRNA。我们也没有用该试剂盒测试过果蝇样本。

5S和5.8S序列更具多样性，因此效果好的探针所使用的物种范围可能会较小：
5S： 0至1个错配：人，小鼠，大鼠，青蛙，鸡，果蝇，线虫， 拟南芥，玉米
5.8S： 0错配：人, 小鼠，大鼠

With regard to species specificity, the following organisms have 0 mismatches for the LSU/SSU probes (LSU (28S) / SSU (18S) Probe perfect matches). This means the probes will remove the 18S and 28S sequences from the following species.

- Species from the Land: Human, mouse, rat, frog, rabbit, cow, pig, chicken, Drosophila,* Caenorhabditis elegans, midge, mosquito, yeast (Saccharomyces cerevisiae, Schizosaccharomyces pombe)
- Species from the Sea: Sea squirt, coelacanth, lancelet, eel, salmon, sturgeon, ratfish, lamprey, brown plankton, zooplankton
- Plants: Arabidopsis thaliana, Brassica napus, rice, tomato, Humulus lupulus, Zea mays, wheat, soybean, pine, aspen

* The 28S and 18S probes in this kit respectively align 100% to the Drosophila 18S and 28S rRNA sequence. However, there are reports suggesting Drosophila (insect) 28S rRNA split into 2 smaller fragments co-migrating with 18S. The exact split site is not identified yet. But based on customer feedback, this kit does not remove the split 28S rRNA completely. We have not tested Drosophila samples with this kit.

The 5S and 5.8S sequences are more divergent, and thus the best probes possible have reduced species breadth:
- 5S: zero to 1 mismatch: human, mouse, rat, frog, chicken, Drosophila, C. elegans, A. thaliana, Z. mays
- 5.8S: zero mismatches: human, mouse, rat