A signal from inside the peroxisome initiates its division by promoting the remodeling of the peroxisomal membrane.
AuthorsGuo T, Gregg C, Boukh-Viner T, Kyryakov P, Goldberg A, Bourque S, Banu F, Haile S, Milijevic S, San KH, Solomon J, Wong V, Titorenko VI
JournalJ Cell Biol
PubMed ID17438077
'We define the dynamics of spatial and temporal reorganization of the team of proteins and lipids serving peroxisome division. The peroxisome becomes competent for division only after it acquires the complete set of matrix proteins involved in lipid metabolism. Overloading the peroxisome with matrix proteins promotes the relocation of acyl-CoA ... More
Cutting edge: myeloid complement C3 enhances the humoral response to peripheral viral infection.
AuthorsVerschoor A, Brockman MA, Knipe DM, Carroll MC
JournalJ Immunol
PubMed ID11509581
HSV-1 is the causative agent of cutaneous lesions, commonly referred to as cold sores. Primary exposure to the virus ordinarily occurs through the periphery, in particular through abraded skin or mucosal membranes. Under certain circumstances (e.g., in neonatals or AIDS patients), the infection becomes disseminated, often with severe consequences. Spread ... More
Authorsvan Raaij EJ, ten Berge D, Hage W, Brouwer A, Meijlink F, Maintz JB, Verbeek FJ
JournalCytometry
PubMed ID11598942
BACKGROUND: Cell proliferation is often studied using the incorporation of bromodeoxyuridine (BrdU). Immunohistochemical staining is then used to detect BrdU in the nucleus. To circumvent the observer bias and labor-intensive nature of manually counting BrdU-labeled nuclei, an automated topographical cell proliferation analysis method is developed. METHODS: Sections stained with fluorescein-labeled ... More
SNAREs and epithelial cells.
AuthorsWeimbs T, Low SH, Li X, Kreitzer G
JournalMethods
PubMed ID12798133
SNARE proteins control the membrane fusion events of membrane trafficking pathways. Work in epithelial cells has shown that polarized trafficking to the apical and basolateral plasma membrane domains requires different sets of SNAREs, suggesting a mechanism that contributes to the overall specificity of polarized trafficking and, perhaps, the formation and ... More