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View additional product information for StemPro™ Accutase™ Cell Dissociation Reagent - FAQs (A1110501)
24 product FAQs found
我们推荐您使用EDTA传代PSCs。如需接种定形内胚层诱导用的细胞，我们推荐您使用StemPro Accutase细胞解离试剂形成小细胞团块，使用Gibco TrypLE酶将细胞分散为单细胞。
人体ES细胞一般可通过以下方法进行鉴定：经典型的形态（它们以致密的细胞团形式生长 生长为紧密聚集的小型细胞团，细胞小并具有高核质比）；表面标志物的表达；干细胞特异性基因表达的RT-PCR检测（如Oct3/4，Sox2和Nanog）；碱性磷酸酶染色和端粒酶活性检测。最为常用的人ES细胞特异性表面标志物包括发育阶段特异性的胚胎抗原SSEA-3和SSEA-4。其它的ES细胞特异性的其它表面抗原还包括TRA-1-60和TRA-1-81。（Science 282:1145 (1998)）。
人胚胎干细胞源自人类囊胚内细胞团, 是通过使用兔抗血清检测BeWO细胞（人滋养层细胞系）抗血清的通过免疫外科法手段来进行分离的（Science 282:1145 (1998)）。
胚胎干（ES）细胞是源自早期哺乳动物胚胎的细胞，能够在体外进行无限的未分化增殖，同时保留分化为各类成体组织（包括生殖细胞）的潜能。ES细胞的多能性通常在体外通过以下方法来证明：将ES细胞诱导分化为类拟胚体，并以谱系特异性的标志物检测三个体胚层（内胚层，中胚层和外胚层）中分化细胞的谱系特异性标志物分化情况，或将这些细胞注射到免疫缺陷型小鼠中，以检测畸胎瘤中产生的细胞类型。
请通过此选择列表(https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html)来比较我们的细胞解离试剂。
可用的酶包括：Gibco TrypLE Express，Gibco TrypLE Select或Gibco StemPro Accutase 细胞解离试剂（Cell Dissociation Reagent）。
Gibco TrypLE Select酶（Gibco TrypLE Select Enzyme，货号 12563011）与Gibco StemPro Accutase细胞解离试剂（货号 A1110501）已测试过均能兼容Gibco Essential 8 Flex培养基。
No. However, although StemPro Accutase is gentle on cells, the optimal time for dissociation should be empirically determined for your specific cell type and application.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
No. StemPro Accutase is supplied as a convenient, ready to use reagent. No dilution is required prior to use.
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No. StemPro Accutase is gentle enough that only dilution of the reagent with DPBS or media is required to stop the dissociation activity.
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We recommend storing StemPro Accutase Cell Dissociation Reagent at -5 to -20 degrees C, protected from light. We do not recommend subjecting it to more than 3-4 freeze-thaws. When stored at 2 to 8 degrees C, the reagent is stable for up to 2 years.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Yes. If StemPro Accutase is exposed to 37 degrees C just until complete thawing is achieved, it still can be used. However, this could result in decreased enzyme activity and it would take more time to get full dissociation. If you observe this, consider starting with a new bottle. Please note that if a bottle of StemPro Accutase is kept at 37 degrees C for more than one hour, it will lose its activity.
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Yes, as long as the StemPro Accutase is still cool to the touch, it should be okay to use.
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Yes. We recommend thawing it at room temperature or overnight at 4 degrees C to get complete thawing. Otherwise, place in the freezer and refreeze.
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Yes. During the freezing procedure or shipping, uneven color can be observed. However, this will not compromise the activity or performance of the enzyme. Just make sure to mix the solution by inverting the tube or bottle to get an even color before use.
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Yes. StemPro Accutase has been tested to dissociate spheres of neural progenitors (neurospheres).
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We recommend using StemPro Accutase wherever gentle and efficient dissociation is needed. We have also validated StemPro Accutase for use with hESC grown in StemPro hESC SFM and for NSC grown in StemPro NSC SFM. All the tests resulted in great recovery of cells without losing their properties and while maintaining good viability. In addition, the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/5029_A1110501_Accutase.pdf) lists examples of cell lines where the application has been validated and published.
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We recommend using EDTA for passaging PSCs. For seeding cells for definitive endoderm induction, we recommend using StemPro Accutase Cell Dissociation Reagent for small clumps and Gibco TrypLE Enzyme for singularized cells.
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Human ES cells are generally characterized by their typical morphology (they grow as tightly packed clusters of small cells with high ratio of nucleus to cytoplasm); surface marker expression; RT-PCR detection of stem cell-specific gene expression (such as Oct3/4, Sox2, and Nanog); alkaline phosphatase staining, and telomerase activity assay. The most commonly used ES specific surface markers include stage-specific embryonic antigens SSEA-3 and SSEA-4 for human ES cells. Other ES-specific surface antigens also include TRA-1-60 and TRA-1-81. (Science 282:1145 (1998).
Human ES cells are derived from human blastocyst inner cell masses, isolated by immunosurgery with rabbit antiserum to BeWO cells (a human trophoblast cell line) (Science 282:1145 (1998)).
Embryonic stem (ES) cells are derived from the early mammalian embryo and are capable of unlimited, undifferentiated proliferation in vitro while maintaining their potential to differentiate into a wide of range of adult tissues including germ cells. The pluripotency of the ES cells is normally demonstrated in vitro by inducing ES cells to differentiate into embryoid bodies and checking lineage-specific markers for differentiated cells in three body layers (endo, meso, and ectoderm), or injecting them into immunodeficient mice and determining the cell types produced in the teratomas.
Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).
Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.
Enzymes that can be used include: Gibco TrypLE Express Enzyme, Gibco TrypLE Select Enzyme, or Gibco StemPro Accutase Cell Dissociation Reagent.
Find additional tips, troubleshooting help, and resources within our Extracellular Matrices (ECM) Support Center.
Gibco TrypLE Select Enzyme (Cat. No. 12563011) and Gibco StemPro Accutase Cell Dissociation Reagent (Cat. No A1110501) have been tested with Essential 8 Flex Medium.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.