A useful approach to total analysis of RISC-associated RNA.
AuthorsHayashida Y, Nishibu T, Inoue K, Kurokawa T,
JournalBMC Res Notes
PubMed ID19706194
'ABSTRACT: BACKGROUND: Identifying the endogenous RNA induced silencing complex(RISC)-associated RNAs is essential for understanding the cellular regulatory networks by miRNAs. Recently, isolation of RISC-associated mRNAs using antibody was reported, but their method needs a large amount of initial materials. We tried to improve the protocol and constructed an efficient and ... More