High-performance liquid chromatographic determination of biotin in biological materials after crown ether-catalyzed fluorescence derivatization with panacyl bromide.
AuthorsRehner GI, Stein J
JournalMethods Enzymol
PubMed ID9211280
High-performance liquid chromatographic determination of biotin in biological materials after crown ether-catalyzed fluorescence derivatization with panacyl bromide.
AuthorsStein J, Hahn A, Lembcke B, Rehner G
JournalAnal Biochem
PubMed ID1534471
This paper reports a high-performance liquid chromatographic (HPLC) technique to determine biotin in biological samples. Biotin and the internal standard dethiobiotin are converted into fluorescent derivatives by using panacyl bromide [p-(9-anthroyloxy)phenacyl bromide] as a fluorescence label. Biotin is extracted from biological tissue with trichloroacetic acid and the extract is purified ... More
Determination of prostaglandins and thromboxane in whole blood by high-performance liquid chromatography with fluorimetric detection.
AuthorsEngels W, Kamps MA, Lemmens PJ, van der Vusse GJ, Reneman RS
JournalJ Chromatogr
PubMed ID3410908
'A highly sensitive and specific assay for the quantitation of prostaglandins (PGs) such as PGE1, PGE2, PGF1 alpha, PGF2 alpha, 6-keto-PGF1 alpha, and including thromboxane B2, is described. The method involves the addition of PGF1 alpha and PGE1 as the internal standards, extraction from whole blood and purification by silica ... More
Separation and quantification of PGE3 following derivatization with panacyl bromide by high pressure liquid chromatography with fluorometric detection.
AuthorsHawkes JS, James MJ, Cleland LG
JournalProstaglandins
PubMed ID1796150
'Separation of prostaglandin E3 (PGE3) from prostaglandin E2 (PGE2) and prostaglandin E1 (PGE1) was achieved following derivatization with p-(9-anthroyloxy)phenacyl bromide (panacyl bromide). The eicosanoid esters were analysed by reverse phase high pressure liquid chromatography with fluorometric detection (excitation 360nm and emission 470nm). Human, rat and mouse adherent cells were incubated ... More
Detection of prostaglandins by high-performance liquid chromatography after conversion to p-(9-anthroyloxy)phenacyl esters.
AuthorsSalari H, Yeung M, Douglas S, Morozowich W
JournalAnal Biochem
PubMed ID3479910
'p-(9-Anthroyloxy)phenacyl bromide (panacyl bromide) undergoes rapid reaction with the carboxyl group of prostaglandins in the presence of N,N-diisopropylethylamine in acetonitrile-tetrahydrofuran (4:1). The resulting prostaglandin panacyl esters are strongly uv absorbing with a lambda max at 253 nm and an epsilon of 174,280 in acetonitrile. The lower limit of detection of ... More
High performance liquid chromatographic determination of thromboxane B2 in human serum as a methoxime-panacylester derivative.
AuthorsPullen RH, Howell JA, Cox JW
JournalProstaglandins Leukot Med
PubMed ID3432306
'A high performance liquid chromatographic (HPLC) method was developed to measure thromboxane B2 (TxB2) levels in human serum. Serum samples (2 mL) were extracted using solid phase extraction columns in a C 18/silica mode sequencing approach. The internal standard, 6-ketoprostaglandin F1 alpha, was added to the serum extracts. The eicosanoids ... More
Fluorescent/ultraviolet absorbing ester derivative formation and analysis of eicosanoids by high-pressure liquid chromatography.
AuthorsWatkins WD, Peterson MB
JournalAnal Biochem
PubMed ID6959545
Picogram measurement of prostaglandin E2 synthesis by gastric mucosa by high-performance liquid chromatography.
AuthorsStein TA, Angus L, Borrero E, Auguste LJ, Wise L
JournalJ Chromatogr
PubMed ID3470299
Prostaglandin biosynthesis by gastric mucosa was determined by a 1-min incubation, solvent extraction, and reaction with panacyl bromide. The prostaglandin ester was measured by normal-phase high-performance liquid chromatography. The prostaglandin E2 levels of normal gastric mucosa of rats and swine were 90.6 +/- 31.0 and 79.8 +/- 39.8 pg/min/mg tissue, ... More
Labeling of free carboxyl groups.
AuthorsYasaka Y, Tanaka M
JournalJ Chromatogr B Biomed Appl
PubMed ID7820274
The latest trends in the labeling of free carboxyl groups for high-performance liquid chromatography are reviewed. The labeling reagents for fluorescence detection are mainly discussed according to their reaction type (or functional group). Attention is also paid to the reagents used for ultraviolet detection and for enantiomeric separation. The reactivity ... More
Ultraviolet and fluorescence derivatization reagents for carboxylic acids suitable for high performance liquid chromatography: a review.
AuthorsMukherjee PS, Karnes HT
JournalBiomed Chromatogr
PubMed ID8879524
Pre- and postcolumn derivatization with a suitable chromophore or fluorophore have often been utilized to enhance the sensitivity and selectivity of detection for analytes possessing a carboxyl group. The major classes of UV and fluorescent derivatization reagents include the coumarin analogues, alkyl halides, diazoalkanes, and amines. The derivatization reaction conditions, ... More
Measurement of prostaglandin G/H synthase and lipoxygenase activity in the stomach wall by HPLC.
AuthorsStein TA, Bailey B, Auguste LJ, Wise L
JournalBiotechniques
PubMed ID1905560
A method for the determination of prostaglandin G/H synthase and lipoxygenase activities in tissues was developed and employed with rat gastric mucosa samples. Tissues and microsomes were incubated in a buffer containing nonionic detergent and 1.32 mM arachidonic acid for 10 min. Following extraction with ethyl acetate, the oxidation products ... More
High-performance liquid-chromatographic assay for prostaglandins with the use of p-(9-anthroyloxy)phenacyl bromide.
AuthorsStein TA, Angus L, Borrero E, Auguste LJ, Wise L
JournalJ Chromatogr
PubMed ID3476489
Gastric mucosa of rats and swine was incubated in buffer for 1 min to produce prostaglandins (PGs). After extraction and derivatization with p-(9-anthroyloxy)phenacyl bromide, the prostaglandin esters were determined by high-performance liquid chromatography. A 20-microliter sample was injected into a microparticulate silica gel column with a mobile phase of dichloromethane-acetonitrile-methanol ... More
Fluorescence labeling and quantification of oxygen-containing functionalities on the surface of single-walled carbon nanotubes.
AuthorsDementev N, Feng X, Borguet E,
JournalLangmuir
PubMed ID19563231
Fluorescence labeling of surface species (FLOSS) was applied to identify and determine the concentration of oxygen-containing functionalities on single-walled carbon nanotubes (SWCNTs), subjected to two different purification processes (air/HCl and nitric acid treatments) and compared to as-received (nonpurified) SWCNTs. The fluorophores were selected for their ability to covalently bind, with ... More
Separation and quantification of prostaglandins E1 and E2 as their panacyl derivatives using reverse phase high pressure liquid chromatography.
AuthorsKrakauer KA, Williamson PK, Baker DG, Zurier RB
JournalProstaglandins
PubMed ID3099332
Separation and quantification of prostaglandin E1 (PGE1) and prostaglandin E2 (PGE2) were achieved using reverse phase high performance liquid chromatography (HPLC). Panacyl bromide (p-(9-anthroyloxy)phenacyl bromide) (PAB) derivatives of PGE2 and PGE1 were prepared. Reverse phase HPLC using a linear gradient of 56% to 80% acetonitrile in water containing 0.10% acetic ... More
Fluorogenic reactions for biomedical chromatography.
AuthorsOhkura Y, Kai M, Nohta H
JournalJ Chromatogr B Biomed Appl
PubMed ID7820282
A number of fluorogenic reactions, which have been used for HPLC detection systems by means of pre- and/or postcolumn derivatization, are surveyed with respect to both sensitivity and selectivity for the determination of biomedically important substances. For the derivatization of the substances, two types of fluorogenic reactions, fluorescence-generating and fluorescence-tagging, ... More
Arachidonic acid protection of rat mucosa against stress ulceration.
AuthorsAuguste LJ, Angus L, Stein TA, Wise L
JournalJ Surg Res
PubMed ID3474479
To evaluate the effect of arachidonic acid (AA), a prostaglandin precursor, on the mucosal level of PGE2 and its possible protective role against stress ulcerations, 40 Holtzman rats were divided into four groups: Group I intragastrically receiving 1 ml of normal saline (NS); Group II, NS pretreatment followed by stress; ... More
Determination of (15R)- and (15S)-15-methylprostaglandin E2 in human plasma with picogram per milliliter sensitivity by column-switching high-performance liquid chromatography.
AuthorsPullen RH, Cox JW
JournalJ Chromatogr
PubMed ID4066871
(15R)-15-Methylprostaglandin E2 (PGE2) is a pro-drug under evaluation for the treatment of acute upper gastrointestinal hemorrhage and gastrointestinal cytoprotection. It is converted in acid (e.g., gastric fluid) to its active 15S epimer. Both epimers are found in human plasma at low pg/ml levels following oral dosing. A high-performance liquid chromatographic ... More