'A nonimmune library of 10(9) human antibody scFv fragments has been cloned and expressed on the surface of yeast, and nanomolar-affinity scFvs routinely obtained by magnetic bead screening and flow-cytometric sorting. The yeast library can be amplified 10(10)-fold without measurable loss of clonal diversity, allowing its effectively indefinite expansion. The ... More
Fluorescence biosensing strategy based on energy transfer between fluorescently labeled receptors and a metallic surface.
AuthorsPérez-Luna VH, Yang S, Rabinovich EM, Buranda T, Sklar LA, Hampton PD, López GP
JournalBiosens Bioelectron
PubMed ID11742737
'A new fluorescence-based biosensor is presented. The biosensing scheme is based on the fact that a fluorophore in close proximity to a metal film (<100 A) experiences strong quenching of fluorescence and a dramatic reduction in the lifetime of the excited state. By immobilizing the analyte of interest (or a ... More