Collagen I, Coated Plate, 6 well - FAQs

View additional product information for Collagen I, Coated Plate, 6 well - FAQs (A1142801)

10 product FAQs found

使用薄层I型胶原包被的平板时，应使用何种试剂来解离细胞？

可用的酶包括：Gibco TrypLE  Express，Gibco TrypLE  Select或Gibco StemPro Accutase 细胞解离试剂（Cell Dissociation Reagent）。

培养于I型胶原的最常见细胞类型有哪些？

以下细胞类型通常培养于I型胶原中：

- 内皮细胞（包括HUVEC）
- 成纤维细胞
- 肝细胞
- 软骨细胞
- 破骨细胞
- 成骨细胞
- 角质形成细胞
- 角膜上皮

I型胶原应如何使用？

其关键应用列举如下：

- I型胶原能够在体外诱导微血管内皮细胞形成纺锤状形态，并排列为类似实线的聚集体。在I型胶原上进行培养时，血管内皮细胞也能够形成类似血管的管状结构。因此，I型胶原也可用于体外的血管生成实验。
- 乳腺癌干细胞在I型胶原上培养时可发生分化。
- I型胶原同样已被用于原代结肠癌细胞系的培养；小鼠肝脏前体细胞和大鼠的胰岛细胞也可培养于3D的I型胶原中。
- I型胶原可在细胞侵润实验分析中作为屏障，也已被用于细胞粘附的相关研究。

Gibco Geltrex 基质，胶原，AlgiMatrix 3D培养系统和CTS Cellstart基质之间的一般性区别是什么？

Gibco Geltrex 基质与胶原（大鼠尾与牛）可作为包被溶液或3D凝胶基质来使用。CELLstart 底物作为一种不含异外源性物质的包被基质，专为ESC的相关应用而研发（作为动物源性的Gibco Geltrex 基质或动物源性的Matrigel 基质的一种替代品底物）。AlgiMatrix 基质是为一种3D支架类的基质，它不支持并不为细胞提供粘附的基础，但而是为细胞球生长提供一个良好的生长环境，后续应用中也可方便易于细胞球的收取操作用于后续应用。

Do I need to use all plates from the Collagen I, Coated Plate, 6 well, (Cat. No. A1142801) package at once or can I store the unused plates? If so, for how long can they be stored?

The unused plates can be resealed in the foil bag and stored for the remaining shelf life of the product.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the dimensions for the Collagen I, Coated Plate, 6 well (Cat. No. A1142801)?

The dimensions for the Collagen I, Coated Plate, 6 well are as follows:
- Height: 14.4 mm
- Length: 127.76 mm
- Width: 85.48 mm

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What can be used to detach cells from plates when coated with a thin layer of collagen I?

Enzymes that can be used include: Gibco TrypLE Express Enzyme, Gibco TrypLE Select Enzyme, or Gibco StemPro Accutase Cell Dissociation Reagent.

Find additional tips, troubleshooting help, and resources within our Extracellular Matrices (ECM) Support Center.

What cell types are most commonly cultured in collagen I?

The following cell types are commonly cultured in collagen I:

- Endothelial cells (including HUVEC)
- Fibroblasts
- Hepatocytes
- Chondrocytes
- Osteoclasts
- Osteoblasts
- Keratinocytes
- Corneal epithelium

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How is collagen I used?

The key applications include the following:

- Collagen I induces microvascular endothelial cells to adopt a spindle-shaped morphology, in vitro, and to align into solid cord-like assemblies. Vascular endothelial cells can also form vessel-like, tubular structures when cultured on collagen I. Collagen I can be used for in vitro angiogenesis assays.
- Breast cancer stem cells can undergo differentiation when cultured on collagen I.
- Collagen I has been used for the culture of primary colon carcinoma cell lines; mouse liver progenitor cells have been cultured in 3D collagen I and rat pancreatic islets.
- Collagen I can act as a barrier in cell invasion assays, and has been used to study cell adhesion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are some general differences between Gibco Geltrex Matrix, collagen, AlgiMatrix 3D Culture System, and CTS Cellstart Substrate?

Gibco Geltrex Matrix and collagen (rat tail and bovine) can be used as either a coating solution or a 3D gel matrix. CELLstart Substrate was developed to be used as a xeno-free coating matrix for only ESC applications (as a substitute for Gibco Geltrex Matrix or Matrigel Matrix, which are of animal origin). AlgiMatrix Matrix is a 3D scaffold-type matrix that does not support cell attachment but does provide a good environment for growing spheroids that can be easily harvested for downstream applications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.