CD FortiCHO™ Medium

CD FortiCHO™ medium is specifically designed to offer the highest batch and fed-batch culture performance and yield with recombinant CHORead more

Catalog Number	Quantity
A1148301	1 L

Catalog number A1148301

Price (CNY)

2,398.00

Add to cart

Quantity:

Price (CNY)

2,398.00

Add to cart

CD FortiCHO™ medium is specifically designed to offer the highest batch and fed-batch culture performance and yield with recombinant CHO cells in a chemically defined environment. The protein-free, animal origin component-free formulation provides consistency and helps reduce the need to screen for adventitious agents. With CD FortiCHO™ medium cell cultures last 2+ days longer than competitive alternatives, which means decreased HCP (Host Cell Protein) concentration for removal in downstream purification.

CD FortiCHO™ medium is fortified to support maximal recombinant CHO cell growth and protein expression in suspension batch culture, offering 120% greater growth and production than select competitive media with simple glucose supplementation. CD FortiCHO™ medium can provide up to 75% greater fed-batch growth and production over glucose-only fed-batch culture for selected cell lines and applications when used with CD EfficientFeed™ C nutrient supplement.

When to Use CD FortiCHO™ Medium
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing

When to Consider Another Gibco™ Medium

Consider CD OptiCHO™ medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky,” i.e. hard to grow or adapt

Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)

Consider CD DG44 when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)

Dry Media Formulations
Dynamis AGT Medium is the comparable dry format for scale up.

Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco™ cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell LineCHO

Concentration1 X

Endotoxin LevelLow

FormatBottle

Included AntibioticsNo antibiotics

Product LineGibco™

Product TypeCD OptiCHO AGT Medium

Quantity1 L

Shipping ConditionRoom Temperature

ClassificationAnimal Origin-free, Chemically-defined, Protein-free, Serum-free

Culture TypeSuspension Cell Culture

FormLiquid

With AdditivesSodium Pyruvate, Sodium Bicarbonate

Without AdditivesNo Glutamine, No Phenol Red

Unit SizeEach

Contents & Storage

Storage:
Store in the dark at +2 to +8°C. Guaranteed stable for 12 months when properly stored.

Frequently asked questions (FAQs)

How do I adapt my cells to serum-free medium?

Cells can be adapted by Sequential or Direct Adaptation. Suggested protocols for each are below, and you can also find more information by searching "Adaptation of Cell Cultures to a Serum-Free Medium" from our website home page.

SEQUENTIAL ADAPTATION
1) Subculture the cells growing in serum-supplemented medium into a 25%:75% mixture of SFM and serum supplemented medium.
2) When the cell density is 5 x 10E5 cells/ml, subculture the cells into a 50%:50% mixture of SFM and serum supplemented medium at a cell density 2.5 x 10E5 to 3 x 10E5 cells/ml.
3) Continue to subculture after the cell density 5 x 10E5 cells/ml in gradually increasing proportions of SFM until the serum is ~0.1% with about 85% cell viability.
4) Subculture the cells into SFM with an innoculum of 2.5 x 10E5 to 3 x 10E5 cells/ml.
5) When the cell density is 1 x 10E6 to 3 x 10E6 cells/ml (4 to 6 days post planting) subculture the cells again.
6) Stock cultures of SFM adapted cells should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.

DIRECT ADAPTATION
Some cells can be directly adapted from serum-containing medium to SFM. For direct adaptation, the cell innoculum should be 1.5 x 10E5 to 3 x 10E5 cells/ml.
Cells should be subcultured when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml. Cells are fully adapted to SFM when the cell density is 2 x 10E6 to 4 x 10E6 cells/ml after 4 to 7 days in culture.
Stock cultures of cells adapted to SFM should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.

Why is it necessary to gradually adapt the cells to serum-free medium?

Some cells, such as insect cells, are sensitive to changes in their medium. By sequentially adapting cells, the medium is changed with minimal effects on cell growth.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.