Molecular amplifiers: synthesis and functionalization of a poly(aminopropyl)dextran bearing a uniquely reactive terminus for univalent attachment to biomolecules.
AuthorsMann JS, Huang JC, Keana JF
JournalBioconjug Chem
PubMed ID1381224
'The synthesis and characterization of the versatile dextran-based molecular amplifier 6 is described. Dextran (Mr = 40,200) was selectively monofunctionalized in high yield at its reducing terminus via reductive amination with 2-(4-nitrophenyl)ethylamine to give 1. The nitro group in 1 serves as a masked amino group which is eventually converted ... More
Protein rotational dynamics investigated with a dual EPR/optical molecular probe. Spin-labeled eosin.
AuthorsCobb CE, Hustedt EJ, Beechem JM, Beth AH
JournalBiophys J
PubMed ID7682451
'An acyl spin-label derivative of 5-aminoeosin (5-SLE) was chemically synthesized and employed in studies of rotational dynamics of the free probe and of the probe when bound noncovalently to bovine serum albumin using the spectroscopic techniques of fluorescence anisotropy decay and electron paramagnetic resonance (EPR) and their long-lifetime counterparts phosphorescence ... More