The mechanisms of inhibition of anion exchange in human erythrocytes by 1-ethyl-3-[3-(trimethylammonio)propyl]carbodiimide.
AuthorsWerner PK, Reithmeier RA
JournalBiochim Biophys Acta
PubMed ID2454665
'Treatment of human erythrocytes with the membrane-impermeant carbodiimide 1-ethyl-3-[3-(trimethylammonio)propyl]carbodiimide (ETC) in citrate-buffered sucrose leads to irreversible inhibition of phosphate-chloride exchange. The level of transport inhibition produced was dependent on the concentration of citrate present during treatment, with a maximum of approx. 60% inhibition. [14C]Citric acid was incorporated into Band 3 ... More
Molecular characterization of the band 3 protein from Southeast Asian ovalocytes.
AuthorsSarabia VE, Casey JR, Reithmeier RA
JournalJ Biol Chem
PubMed ID8486716
'Southeast Asian ovalocytosis (SAO) is a hereditary form of elliptocytosis resulting in rigid, oval-shaped erythrocytes resistant to invasion by malaria parasites. The molecular defect is due to deletion of codons 400-408, encoding a 9-amino-acid sequence located at the boundary between the cytosol and the first transmembrane segment in Band 3, ... More
Location of the stilbenedisulfonate binding site of the human erythrocyte anion-exchange system by resonance energy transfer.
AuthorsRao A, Martin P, Reithmeier RA, Cantley LC
JournalBiochemistry
PubMed ID497152
'The stilbenedisulfonate inhibitory site of the human erythrocyte anion-exchange system has been characterized by using serveral fluorescent stilbenedisulfonates. The covalent inhibitor 4-benzamido-4''-isothiocyanostilbene-2,2''-disulfonate (BIDS) reacts specifically with the band 3 protein of the plasma membrane when added to intact erythrocytes, and the reversible inhibitors 4,4''-dibenzamidostilbene-2,2''-disulfonate (DBDS) and 4-benzamido-4''-aminostilbene-2,2''-disulfonate (BADS) show a ... More
Self-association of Band 3, the human erythrocyte anion exchanger, in detergent solution.
AuthorsVince JW, Sarabia VE, Reithmeier RA
JournalBiochim Biophys Acta
PubMed ID9218560
'Dimeric Band 3 purified in n-dodecyl octaethyleneglycol (C12E8) underwent an irreversible, temperature-dependent association, resulting in a complex with a Stokes radius slightly larger than a native tetramer, before forming a higher molecular weight aggregate. Self-association occurred with a half-time of about 1 h at 37 degrees C but did not ... More
Identification, purification, and characterization of a stilbenedisulfonate binding glycoprotein from canine kidney brush border membranes. A candidate for a renal anion exchanger.
AuthorsPimplikar SW, Reithmeier RA
JournalJ Biol Chem
PubMed ID3346257
'Canine renal brush border membrane proteins that bind stilbenedisulfonate inhibitors of anion exchange were identified by affinity chromatography. A 130-kDa integral membrane glycoprotein from brush border membrane was shown to bind specifically to 4-acetamido-4''-isothiocyanostilbene-2,2''-disulfonate immobilized on Affi-Gel 102 resin. The bound protein could be eluted effectively with 1 mM 4-benzamido-4''-aminostilbene-2,2''-disulfonate ... More
Characterization of the stilbenedisulfonate binding site of the band 3 polypeptide of human erythrocyte membranes.
AuthorsLieberman DM, Reithmeier RA
JournalBiochemistry
PubMed ID6615815
A diaminostilbene dye as a hydrophobic probe for proteins.
AuthorsKotaki A, Naoi M, Yagi K
JournalBiochim Biophys Acta
PubMed ID5103023
Affinity chromatography of Band 3, the anion transport protein of erythrocyte membranes.
AuthorsPimplikar SW, Reithmeier RA
JournalJ Biol Chem
PubMed ID3733694
Affinity chromatography of Band 3 was performed using a series of affinity matrices synthesized with various inhibitor ligands and spacer arms. Hydrophilic spacer arms greater than four atoms in length were essential for Band 3 binding. An affinity resin prepared by reacting 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonate (Ki = 10 microM) with Affi-Gel 102 ... More
Human erythrocyte band 3 polymorphism (band 3 Memphis): characterization of the structural modification (Lys 56----Glu) by protein chemistry methods.
AuthorsYannoukakos D, Vasseur C, Driancourt C, Blouquit Y, Delaunay J, Wajcman H, Bursaux E
JournalBlood
PubMed ID1678289
Band 3 variants occur rather frequently in different populations. Based on sodium dodecyl sulfate (SDS)-polyacrylamide electrophoretic properties, a widespread polymorphism (band 3 Memphis) has been previously described. It corresponds to a protein that has been hypothesized to be elongated in its N-terminal cytoplasmic domain. Band 3 from a heterozygote subject ... More
Characterization of matrix-bound Band 3, the anion transport protein from human erythrocyte membranes.
AuthorsBoodhoo A, Reithmeier RA
JournalJ Biol Chem
PubMed ID6693394
Band 3 (Mr = 95,000), the anion transport protein of human erythrocyte membranes exists primarily as a dimer in solutions of nonionic detergents such as octaethylene glycol mono-n-dodecyl ether (C12E8). The role of the oligomeric structure of Band 3 in the binding of [14C]4-benzamido-4'-aminostilbene-2,2'-disulfonate (BADS), an inhibitor of anion transport ... More
Hydrodynamic properties of human erythrocyte band 3 solubilized in reduced Triton X-100.
AuthorsTaylor AM, Boulter J, Harding SE, Cölfen H, Watts A
JournalBiophys J
PubMed ID10096900
The oligomeric state and function of band 3, purified by sulfhydryl affinity chromatography in reduced Triton X-100, was investigated. Size exclusion high-performance liquid chromatography showed that a homogeneous population of band 3 dimers could be purified from whole erythrocyte membranes. The elution profile of band 3 purified from membranes that ... More
Study on coacervation of the repeat pentapeptide model of tropoelastin: effect of cations.
AuthorsKondo M, Nakashima Y, Kodama H, Okamoto K
JournalJ Biochem (Tokyo)
PubMed ID3571212
The effects of various cations on coacervation of the polypentapeptide (Val-Pro-Gly-Val-Gly)n, a sequential model of tropoelastin, were studied by following the turbidity at 300 nm and the fluorescence intensity due to the interaction between the polypentapeptide and a hydrophobic probe (4-benzoylamido-4'-aminostilbene-2,2'-disulfonic acid). In the absence of cations, as the polypentapeptide ... More
Factors determining the conformation and quaternary structure of isolated human erythrocyte band 3 in detergent solution.
AuthorsSchopfer LM, Salhany JM
JournalBiochemistry
PubMed ID1472497
Fluorescence spectroscopy was used to follow the kinetics of covalent binding of DIDS (4,4'-diisothiocyanato-2,2'-stilbenedisulfonate) to isolated band 3 in C12E8. We have discovered a dilution-induced loss in the ability of band 3 monomer to form a covalent adduct with DIDS. The loss in DIDS reactivity with dilution followed a 50:50 ... More
Characterization of BADS-binding proteins in epithelial plasma membranes.
AuthorsPearce SF, Zadunaisky JA
JournalJ Membr Biol
PubMed ID1744902
When a fluorescent stilbene was added to epithelial plasma membrane suspension the emission spectrum showed a broad peak containing overlapping emissions resulting from different adducts. By focusing on a specific emission wavelength a common site having a dissociation constant of approximately 5 microM was calculated in the rat kidney, small ... More
Interaction of hydrophobic probes with the apoenzyme of pig heart lipoamide dehydrogenase.
AuthorsOgasahara K, Koike K, Hamada M, Hiraoka T
JournalJ Biochem (Tokyo)
PubMed ID956145
The interaction of hydrophobic probes, 8-anilinonaphthalene-1-sulfonate (ANS) and 4-benzoylamido-4'-aminostilbene-2, 2'-disulfonate (MBAS), with pig heart lipoamide dehydrogenase [NADH: lipoamide oxidoreductase, EC 1.6.4.3] was investigated. When ANS or MBAS was mixed with the apoenzyme of lipoamide dehydrogenase, the fluorescence quantum yield, of each dye was enhancedd markedly and the emission maxima concurrently ... More
Studies on the interaction of matrix-bound inhibitor with Band 3, the anion transport protein of human erythrocyte membranes.
AuthorsPimplikar SW, Reithmeier RA
JournalBiochim Biophys Acta
PubMed ID3395612
The effect of temperature and chemical modification on the interaction of the human erythrocyte Band 3 protein (the anion transport protein) with 4-acetamido-4'-isothiocyanostilbene 2,2'-disulfonate (SITS; Ki = 10 microM)-Affi-Gel 102 resin was studied. Band 3 binds to the affinity resin in two states; weakly bound, which is eluted by 1 ... More
Molecular characterization of the human erythrocyte anion transport protein in octyl glucoside.
AuthorsWerner PK, Reithmeier RA
JournalBiochemistry
PubMed ID4084527
Band 3 protein, the anion transport protein of the human erythrocyte membrane, was purified in the presence of the nonionic detergent octyl glucoside. A molecular characterization was carried out to investigate whether the native structure of the protein was retained in the presence of this detergent. Band 3 bound octyl ... More
Fluorescent stilbene (BADS) binding proteins in anion-transporting epithelia.
AuthorsPearce SF, Zadunaisky JA
JournalAm J Physiol
PubMed ID2399967
Chloride transport occurs at the interface between the internal and external environments of a cell where chloride uptake or efflux is regulated through a variety of mechanisms that involve cotransport of cations, exchange mechanism with anions, or movement through channels. One of these mechanisms, a chloride-bicarbonate exchange found in the ... More