Amplex™ Red Monoamine Oxidase Assay Kit

The Amplex Red Monoamine Oxidase Assay Kit provides a sensitive and simple fluorometric method for detecting monoamine oxidase activity inRead more

Catalog Number	Quantity
A12214	500 Assays

Catalog number A12214

Price (CNY)

6,650.00

Add to cart

Quantity:

500 Assays

Price (CNY)

6,650.00

Add to cart

The Amplex Red Monoamine Oxidase Assay Kit provides a sensitive and simple fluorometric method for detecting monoamine oxidase activity in purified enzyme preparations at levels as low as 12 μU/mL and in tissue samples with as little as 20 μg protein in a 100 μL assay volume.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodFluorescence

Dye TypeOther Label(s) or Dye(s)

FormatCuvettes, 96-well plate

Quantity500 Assays

Shipping ConditionRoom Temperature

For Use With (Application)Monoamine Oxidase Assay

For Use With (Equipment)Fluorometer, Microplate Reader

Product LineAmplex

Product TypeAmplex Red Assay Kit

Unit SizeEach

Contents & Storage

Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H₂O₂ oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can Amplex Red Assays be performed using cell lysates?

This is not recommended. The presence of endogenous proteases can complicate the assay by degrading the horseradish peroxidase (HRP). Endogenous peroxidases and antioxidants can modify the H₂O₂ required for the reaction, competing with HRP (and catalase) for the substrate.

The Amplex Red Assays are best performed with either purified enzymes or extracted H₂O₂ in a defined buffer system, extracellular solutions or body fluids (media, serum, etc.) that do not exhibit high levels of endogenous protease or oxidase activity and do not contain antioxidants.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.