Hexokinase inhibits flux of fluorescently labeled ATP through mitochondrial outer membrane porin.
AuthorsPerevoshchikova IV, Zorov SD, Kotova EA, Zorov DB, Antonenko YN,
JournalFEBS Lett
PubMed ID20412805
'Mitochondrial function requires maintaining metabolite fluxes across the mitochondrial outer membrane, which is mediated primarily by the voltage dependent anion channel (VDAC). We applied fluorescence correlation spectroscopy (FCS) to study regulation of the VDAC functional state by monitoring distribution of fluorescently labeled ATP (BODIPY-FL-ATP) in isolated intact rat liver and ... More
Evidence for major structural changes in subunit C of the vacuolar ATPase due to nucleotide binding.
AuthorsArmbrüster A, Hohn C, Hermesdorf A, Schumacher K, Börsch M, Grüber G
JournalFEBS Lett
PubMed ID15792803
The ability of subunit C of eukaryotic V-ATPases to bind ADP and ATP is demonstrated by photoaffinity labeling and fluorescence correlation spectroscopy (FCS). Quantitation of the photoaffinity and the FCS data indicate that the ATP-analogues bind more weakly to subunit C than the ADP-analogues. Site-directed mutagenesis and N-terminal sequencing of ... More
ATP modulation of excitatory synapses onto interneurons.
AuthorsKhakh BS, Gittermann D, Cockayne DA, Jones A
JournalJ Neurosci
PubMed ID12917379
Inhibitory interneurons play important roles in neuronal circuits, but the synaptic mechanisms that regulate excitatory input onto interneurons remain to be fully understood. We show that ATP-gated presynaptic P2X2 channels facilitate excitatory transmission onto stratum radiatum interneurons but not onto CA1 pyramidal neurons. ATP released endogenously during carbachol-induced oscillations facilitates ... More
Detection of adenylyl cyclase activity using a fluorescent ATP substrate and capillary electrophoresis.
AuthorsCunliffe JM, Sunahara RK, Kennedy RT
JournalAnal Chem
PubMed ID16536404
A capillary electrophoresis laser-induced fluorescence (CE-LIF) assay was developed for detection of adenylyl cyclase (AC) activity using BODIPY FL ATP (BATP) as substrate. In the assay, BATP was incubated with AC and the resulting mixture of BATP and enzyme product (BODIPY cyclic AMP, BcAMP) separated in 5 min by CE-LIF. ... More
Tannin Supplementation Improves Oocyte Cytoplasmic Maturation and Subsequent Embryo Development in Pigs.
Authors
JournalAntioxidants (Basel)
PubMed ID34679729
Single-Cell Transcriptomics of Human Oocytes: Environment-Driven Metabolic Competition and Compensatory Mechanisms During Oocyte Maturation.
Authors
JournalAntioxid Redox Signal
PubMed ID29486586
Single-Molecule Fluorescence Methods to Study Plant Hormone Signal Transduction Pathways.
Authors
JournalFront Plant Sci
PubMed ID29163610
Melatonin Regulates Lipid Metabolism in Porcine Cumulus-Oocyte Complexes via the Melatonin Receptor 2.
Authors
JournalAntioxidants (Basel)
PubMed ID35453372
Melatonin-Nrf2 Signaling Activates Peroxisomal Activities in Porcine Cumulus Cell-Oocyte Complexes.
Authors
JournalAntioxidants (Basel)
PubMed ID33153240
HIV-1 gp120 Promotes Lysosomal Exocytosis in Human Schwann Cells.
Authors
JournalFront Cell Neurosci
PubMed ID31379513
Glycine treatment enhances developmental potential of porcine oocytes and early embryos by inhibiting apoptosis.
Authors
JournalJ Anim Sci
PubMed ID29762687
Effect of Klotho protein during porcine oocyte maturation via Wnt signaling.
Authors
JournalAging (Albany NY)
PubMed ID33176278
Crosstalk between Peroxisomal Activities and Nrf2 Signaling in Porcine Embryos.
Authors
JournalAntioxidants (Basel)
PubMed ID34068072
Arginine Regulates Zygotic Genome Activation in Porcine Embryos Under Nutrition Restriction.