Hibernate™-E Medium - FAQs

查看更多产品信息 Hibernate™-E Medium - FAQs (A1247601)

3 个常见问题解答

在环境浓度的CO2条件下维持/操作神经元，应使用何种培养基？

我们针对此项应用提供了Gibco Hibernate培养基产品。当加入Gibco B-27添加剂与Gibco GlutaMAX-1添加剂时，此培养基能够允许用户在环境CO2条件下操作神经元至少48小时，同时保持其活力；也能够在4°C储存条件下保持脑组织的活力长达一个月的时间。我们提供两种Hibernate培养基：

•Gibco Hibernate-A培养基（货号 A1247501）专为出生后神经元而配制。
•Gibco Hibernate-E培养基（货号 A1247601）专为胚胎神经元而配制。

这两类神经元拥有相同配方而仅在渗透压参数上有所不同。

Can the Hibernate-E Medium (Cat. No. A1247601) be used for plating and growing mouse primary hippocampal neurons?

Hibernate-E medium (Cat. No. A1247601) is designed for the maintenance of embryonic neural tissue and is suitable for short-term maintenance of cultured rat neurons at 37°C in ambient CO2 (up to 2 days) and long-term storage of viable brain tissue at 2 to 8°C (up to a month). However, it is not recommended for the initial plating and growing of neurons.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What medium can be used to maintain/manipulate neurons at ambient CO2?

We offer Gibco Hibernate Medium for this purpose. When supplemented with Gibco B-27 Supplement and Gibco GlutaMAX-1 Supplement, this medium can allow for the manipulation of neurons at ambient CO2 for at least 48 hours while retaining their viability, and preserve viable brain tissue for up to a month when stored at 4 degrees C. Two Hibernate media are offered:

- Gibco Hibernate-A Medium (Cat. No. A1247501) is formulated for postnatal neurons.
- Gibco Hibernate-E Medium (Cat. No. A1247601) is formulated for embryonic neurons.

These two media have the same formulation and differ only in osmolality.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.